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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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January 2012 Volume 40 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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January 2012 Volume 40 Issue 1

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Article

Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning

  • Authors:
    • Fumi Shibata-Minoshima
    • Toshihiko Oki
    • Noriko Doki
    • Fumio Nakahara
    • Shun-Ichiro Kageyama
    • Jiro Kitaura
    • Junya Fukuoka
    • Toshio Kitamura
  • View Affiliations / Copyright

    Affiliations: Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan, Department of Surgical Pathology, Toyama University, Toyama 930-0194, Japan
  • Pages: 93-98
    |
    Published online on: August 19, 2011
       https://doi.org/10.3892/ijo.2011.1173
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Abstract

Multiple mutations contribute to establish cancers. We have searched for potential oncogenes by screening cDNA libraries derived from gastric cancer cell lines, pancreatic cancer cell lines and glioma cell lines, using retrovirus-mediated expression cloning. Two types of interleukin-3 (IL-3)-dependent cell lines, Ba/F3 and HF6, were transduced with the cDNA libraries and several genes that render these cells factor-independent were identified including PIM-1, PIM-2, PIM-3, GADD45B and reproductive homeobox genes on the X chromosome gene F2 (RHOXF2). Although no mutation in these genes was found, these molecules were highly expressed in cancer cell lines and they may play important roles in cell transformation. Among them, we focused on a transcriptional repressor RHOXF2. Transduction of RHOXF2 rendered HF6 cells factor-independent, while knockdown of RHOXF2 inhibited growth of the HGC27 gastric cancer cell line which highly expresses RHOXF2. In addition, RHOXF2-transduced HF6 cells quickly induced leukemia when transplanted into sublethally irradiated mice. Moreover, RHOXF2 is highly expressed in some leukemia cell lines and a variety of human cancer samples including colon and lung cancers. Thus, these results indicate that RHOXF2 is involved in carcinogenesis.

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Copy and paste a formatted citation
Spandidos Publications style
Shibata-Minoshima F, Oki T, Doki N, Nakahara F, Kageyama S, Kitaura J, Fukuoka J and Kitamura T: Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning. Int J Oncol 40: 93-98, 2012.
APA
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J. ... Kitamura, T. (2012). Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning. International Journal of Oncology, 40, 93-98. https://doi.org/10.3892/ijo.2011.1173
MLA
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J., Fukuoka, J., Kitamura, T."Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning". International Journal of Oncology 40.1 (2012): 93-98.
Chicago
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J., Fukuoka, J., Kitamura, T."Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning". International Journal of Oncology 40, no. 1 (2012): 93-98. https://doi.org/10.3892/ijo.2011.1173
Copy and paste a formatted citation
x
Spandidos Publications style
Shibata-Minoshima F, Oki T, Doki N, Nakahara F, Kageyama S, Kitaura J, Fukuoka J and Kitamura T: Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning. Int J Oncol 40: 93-98, 2012.
APA
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J. ... Kitamura, T. (2012). Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning. International Journal of Oncology, 40, 93-98. https://doi.org/10.3892/ijo.2011.1173
MLA
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J., Fukuoka, J., Kitamura, T."Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning". International Journal of Oncology 40.1 (2012): 93-98.
Chicago
Shibata-Minoshima, F., Oki, T., Doki, N., Nakahara, F., Kageyama, S., Kitaura, J., Fukuoka, J., Kitamura, T."Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning". International Journal of Oncology 40, no. 1 (2012): 93-98. https://doi.org/10.3892/ijo.2011.1173
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