Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells

  • Authors:
    • Bo Yin
    • Yang Yang
    • Zhiqiang Zhao
    • Yu Zeng
    • Steven M. Mooney
    • Ming  Li
    • Xuewen Xu
    • Yongsheng Song
    • Bin Wu
    • Zhibo Yang
  • View Affiliations

  • Published online on: January 11, 2011     https://doi.org/10.3892/ijo.2011.901
  • Pages: 1041-1046
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or a potential diagnostic predictor for PCa. In this study, we examined the expression of 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as a unique marker and therapeutic target for PCa SCs. To this end, we isolated the cancer stem cell-like side population (SP) cells from the human PCa cell line DU-145 by a flow cytometry-based SP technique. The isolated DU-145 SP cells comprised a small population of the DU-145 cells. The SP cells had an up-regulation of ATP-binding cassette sub-family G member 2 (ABCG2) which enables these cells to efflux vital dyes and chemotherapeutic drugs. Furthermore, we detected a strong up-regulation of 12-LOX in these DU-145 SP cells compared to the parental DU-145 cells by RT-PCR and Western blot approaches. We also detected 12-LOX overexpression in PCa SCs in human PCa tissue samples by paraffin-section based immunofluorescent 4-channel confocal microscopy. However, no 12-LOX was detected in normal prostate epithelial SCs in normal prostate tissue samples. These multiple lines of evidence support the possibility that 12-LOX may serve as a unique marker and therapeutic target for PCa SCs.

Related Articles

Journal Cover

April 2011
Volume 38 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yin, B., Yang, Y., Zhao, Z., Zeng, Y., Mooney, S.M., Li, M. ... Yang, Z. (2011). Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells. International Journal of Oncology, 38, 1041-1046. https://doi.org/10.3892/ijo.2011.901
MLA
Yin, B., Yang, Y., Zhao, Z., Zeng, Y., Mooney, S. M., Li, M., Xu, X., Song, Y., Wu, B., Yang, Z."Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells". International Journal of Oncology 38.4 (2011): 1041-1046.
Chicago
Yin, B., Yang, Y., Zhao, Z., Zeng, Y., Mooney, S. M., Li, M., Xu, X., Song, Y., Wu, B., Yang, Z."Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells". International Journal of Oncology 38, no. 4 (2011): 1041-1046. https://doi.org/10.3892/ijo.2011.901