Open Access

Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1

  • Authors:
    • Miki Fuse
    • Nijiro Nohata
    • Satoko Kojima
    • Shinichi Sakamoto
    • Takeshi Chiyomaru
    • Kazumori Kawakami
    • Hideki Enokida
    • Masayuki Nakagawa
    • Yukio Naya
    • Tomohiko Ichikawa
    • Naohiko Seki
  • View Affiliations

  • Published online on: January 21, 2011     https://doi.org/10.3892/ijo.2011.919
  • Pages: 1093-1101
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression, primarily at the post-transcriptional level. Growing evidence suggests that miRNAs function as oncogenes or tumor suppressors in human cancers. The down-regulation of miR-145 has been reported in many types of human cancer, including prostate cancer (PC), suggesting that miR-145 functions as a tumor suppressor. Using the PC cell lines, PC3 and DU145, gain-of-function assays revealed that miR-145 transfection inhibited cell proliferation, migration and invasion. Fascin homolog 1 (FSCN1), an actin-bundling protein, is a candidate target gene of miR-145 based on genome-wide gene expression analysis. A luciferase reporter assay showed a significantly decreased signal at two miR-145 target sites at the 3'UTR of FSCN1, suggesting that miR-145 directly regulates FSCN1. In FSCN1 loss-of-function assays, cell growth, migration and invasion were all inhibited, implying that FSCN1 is associated with the progression of PC. The identification of tumor suppressive miRNAs and their target genes could provide new insights into the potential mechanisms of prostate carcinogenesis.

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April 2011
Volume 38 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Fuse, M., Nohata, N., Kojima, S., Sakamoto, S., Chiyomaru, T., Kawakami, K. ... Seki, N. (2011). Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1. International Journal of Oncology, 38, 1093-1101. https://doi.org/10.3892/ijo.2011.919
MLA
Fuse, M., Nohata, N., Kojima, S., Sakamoto, S., Chiyomaru, T., Kawakami, K., Enokida, H., Nakagawa, M., Naya, Y., Ichikawa, T., Seki, N."Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1". International Journal of Oncology 38.4 (2011): 1093-1101.
Chicago
Fuse, M., Nohata, N., Kojima, S., Sakamoto, S., Chiyomaru, T., Kawakami, K., Enokida, H., Nakagawa, M., Naya, Y., Ichikawa, T., Seki, N."Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1". International Journal of Oncology 38, no. 4 (2011): 1093-1101. https://doi.org/10.3892/ijo.2011.919