Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways

  • Authors:
    • Wen-Wen Huang
    • Shih-Wei Ko
    • Huei-Yann Tsai
    • Jing-Gung Chung
    • Jo-Hua Chiang
    • Kuan-Tin Chen
    • Yen-Chen Chen
    • Hung-Yi Chen
    • Yuh-Fung Chen
    • Jai-Sing Yang
  • View Affiliations

  • Published online on: January 24, 2011     https://doi.org/10.3892/ijo.2011.922
  • Pages: 1067-1073
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Abstract

Cantharidin (CTD) is a traditional Chinese medicine and an effective component isolated from blister beetle, and it has been demonstrated to have anticancer, antibiotic, antivirus activities and immune-regulated functions. It has been reported that CTD induces cell cycle arrest and apoptosis in many cancer cell types. However, there are no reports showing that CTD would induce cell cycle arrest and apoptosis in human colorectal cancer colo 205 cells. In this study, we studied colo 205 cells which were treated with CTD and demonstrated its molecular mechanisms in apoptosis. CTD induced growth inhibition, G2/M phase arrest and apoptosis in colo 205 cells. The IC50 is 20.53 µM in CTD-treated colo 205 cells. DAPI/TUNEL double staining and Annexin V assays were used to confirm the apoptotic cell death in colo 205 cells after CTD exposure. CTD caused G2/M arrest, down-regulated CDK1 activity, decreased Cyclin A, Cyclin B, CDK1 and increased CHK1 and p21 protein levels. Colorimetric assays also indicated that CTD triggered activities of casapse-8, -9 and -3 in colo 205 cells. Moreover, CTD increased ROS production and decreased the level of mitochondrial membrane potential (ΔΨm) in colo 205 cells. Consequently, CTD-induced growth inhibition was significantly attenuated by N-acetylcysteine (NAC, a scavenger). CTD stimulated the protein levels of Fas/CD95, the caspase-3 active form, cytochrome c and Bax, but suppressed the protein levels of pro-caspase-8, pro-caspase-9 and Bcl-2, determined by Western blot analysis. Based on our observations, we suggest that CTD is able to induce G2/M phase arrest and apoptosis in colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways.

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April 2011
Volume 38 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Huang W, Ko S, Tsai H, Chung J, Chiang J, Chen K, Chen Y, Chen H, Chen Y, Yang J, Yang J, et al: Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways. Int J Oncol 38: 1067-1073, 2011.
APA
Huang, W., Ko, S., Tsai, H., Chung, J., Chiang, J., Chen, K. ... Yang, J. (2011). Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways. International Journal of Oncology, 38, 1067-1073. https://doi.org/10.3892/ijo.2011.922
MLA
Huang, W., Ko, S., Tsai, H., Chung, J., Chiang, J., Chen, K., Chen, Y., Chen, H., Chen, Y., Yang, J."Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways". International Journal of Oncology 38.4 (2011): 1067-1073.
Chicago
Huang, W., Ko, S., Tsai, H., Chung, J., Chiang, J., Chen, K., Chen, Y., Chen, H., Chen, Y., Yang, J."Cantharidin induces G2/M phase arrest and apoptosis in human colorectal cancer colo 205 cells through inhibition of CDK1 activity and caspase-dependent signaling pathways". International Journal of Oncology 38, no. 4 (2011): 1067-1073. https://doi.org/10.3892/ijo.2011.922