CYP1A1 is a target of miR-892a-mediated post-transcriptional repression

  • Authors:
    • Yeong Min Choi
    • Sungkwan An
    • Eun-Mee Lee
    • Karam Kim
    • Sung Jin Choi
    • Ju Sub Kim
    • Hyun Hee Jang
    • In-Sook An
    • Seunghee Bae
  • View Affiliations

  • Published online on: March 28, 2012     https://doi.org/10.3892/ijo.2012.1418
  • Pages: 331-336
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Abstract

Cytochrome P450 1A1 (CYP1A1) is a member of the cytochrome p450 enzyme family, which is involved in the metabolisms of carcinogenic metabolites, such as benzo(a)pyrene. In this study, we identified miR-892a as a negative regulator of CYP1A1 expression. Luciferase assays revealed a sequence in the 3'-untranslated region of CYP1A1 that displayed a perfect match with miR-892a, and revealed that this sequence was a specific miR-892a target site. The overexpression of miR‑892a inhibited the expression of the CYP1A1 protein, and the miR‑892a antagonist increased CYP1A1 expression. Of note, benzo(a)pyrene, a major inducer of CYP1A1 transcription, decreased the expression of miR-892a. Moreover, the miR-892a-induced CYP1A1 repression inhibited the benzo(a)pyrene-mediated decrease in cell viability. These data provide insight into the CYP1A1 regulatory network.

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July 2012
Volume 41 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Choi YM, An S, Lee E, Kim K, Choi SJ, Kim JS, Jang HH, An I and Bae S: CYP1A1 is a target of miR-892a-mediated post-transcriptional repression. Int J Oncol 41: 331-336, 2012
APA
Choi, Y.M., An, S., Lee, E., Kim, K., Choi, S.J., Kim, J.S. ... Bae, S. (2012). CYP1A1 is a target of miR-892a-mediated post-transcriptional repression. International Journal of Oncology, 41, 331-336. https://doi.org/10.3892/ijo.2012.1418
MLA
Choi, Y. M., An, S., Lee, E., Kim, K., Choi, S. J., Kim, J. S., Jang, H. H., An, I., Bae, S."CYP1A1 is a target of miR-892a-mediated post-transcriptional repression". International Journal of Oncology 41.1 (2012): 331-336.
Chicago
Choi, Y. M., An, S., Lee, E., Kim, K., Choi, S. J., Kim, J. S., Jang, H. H., An, I., Bae, S."CYP1A1 is a target of miR-892a-mediated post-transcriptional repression". International Journal of Oncology 41, no. 1 (2012): 331-336. https://doi.org/10.3892/ijo.2012.1418