miR-155 is up-regulated in primary and secondary glioblastoma and promotes tumour growth by inhibiting GABA receptors

  • Authors:
    • Pietro I. D'Urso
    • Oscar F. D'Urso
    • Carlo Storelli
    • Massimo  Mallardo
    • Cosimo Damiano Gianfreda
    • Antonio Montinaro
    • Antonia  Cimmino
    • Caliandro Pietro
    • Santo Marsigliante
  • View Affiliations

  • Published online on: March 28, 2012     https://doi.org/10.3892/ijo.2012.1420
  • Pages:228-234
0

Abstract

An altered expression of microRNAs (miRNAs) contributes both to the development of cancer and to the progression of the disease. Malignant tumours and tumour cell lines have widespread deregulated expressions of miRNAs compared to normal tissues. In this study, we investigated the expression profiles of 340 mammalian miRNAs in 93 cases of multiform glioblastoma (primary and secondary glioblastoma tumours), by means of DNA microarrays. We show that the expression profiles of 10 miRNAs can distinguish primary from secondary glioblastoma types. Moreover, we found elevated miR-155 levels in primary and secondary glioblastoma tissues as well as in glioblastoma primary cultures. We hypothesised that γ-aminobutyric acid A receptor 1 (GABRA1) is a miR-155 target, and studied the correlation between miR-155 up-regulation and the GABRA1 protein in cultured glioblastoma cells by miRNA silencing. We show that a decrease in miR-155 expression to normal levels restores the expression of GABRA1, making glioblastoma cells sensitive to signals that inhibit cell proliferation mediated by GABRA1. In conclusion, the expression patterns of different miRNAs characterise primary and secondary glioblastomas. The aberrant overexpression of miR-155 contributes to the malignant phenotype of glioblastoma cells removing growth inhibition.

Related Articles

Journal Cover

July 2012
Volume 41 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
D'Urso, P.I., D'Urso, O. ., Storelli, C., Mallardo, M., Gianfreda, C.D., Montinaro, A. ... Marsigliante, S. (2012). miR-155 is up-regulated in primary and secondary glioblastoma and promotes tumour growth by inhibiting GABA receptors. International Journal of Oncology, 41, 228-234. https://doi.org/10.3892/ijo.2012.1420
MLA
D'Urso, P. I., D'Urso, O. ., Storelli, C., Mallardo, M., Gianfreda, C. D., Montinaro, A., Cimmino, A., Pietro, C., Marsigliante, S."miR-155 is up-regulated in primary and secondary glioblastoma and promotes tumour growth by inhibiting GABA receptors". International Journal of Oncology 41.1 (2012): 228-234.
Chicago
D'Urso, P. I., D'Urso, O. ., Storelli, C., Mallardo, M., Gianfreda, C. D., Montinaro, A., Cimmino, A., Pietro, C., Marsigliante, S."miR-155 is up-regulated in primary and secondary glioblastoma and promotes tumour growth by inhibiting GABA receptors". International Journal of Oncology 41, no. 1 (2012): 228-234. https://doi.org/10.3892/ijo.2012.1420