Assessment of EGFR and K-ras mutations in fixed and fresh specimens from transesophageal ultrasound-guided fine needle aspiration in non-small cell lung cancer patients

  • Authors:
    • Paola Ulivi
    • Micaela Romagnoli
    • Elisa Chiadini
    • Gian-Luca Casoni
    • Laura Capelli
    • Carlo Gurioli
    • Wainer Zoli
    • Luca Saragoni
    • Alessandra Dubini
    • Anna Tesei
    • Dino Amadori
    • Venerino Poletti
  • View Affiliations

  • Published online on: April 10, 2012     https://doi.org/10.3892/ijo.2012.1432
  • Pages: 147-152
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Abstract

In non-small cell lung cancer (NSCLC) patients, somatic EGFR and K-ras mutations predict therapeutic effectiveness and resistance, respectively, to EGFR tyrosine kinase inhibitors (TKIs). Transesophageal ultrasound-guided fine needle aspiration (EUS-FNA) is a validated technique for diagnosis and staging of NSCLC. In the present study, we compared the feasibility and reliability of EGFR and K-ras gene mutation analysis in fixed and fresh mediastinal lymph nodes and extra-lymph nodal samples obtained by EUS-FNA in patients suspicious for NSCLC. Thirty-six patients were enrolled into the study. For each patient, DNA was extracted from both fresh samples and fixed cytological smears. Exons 18-21 of EGFR and exon 2 of K-ras were amplified by PCR and mutation status was determined by direct sequencing and pyrosequencing. All cases were eligible for analysis. NSCLC was diagnosed in 32 patients (25 adenocarcinomas and 7 squamous cell carcinomas) and 4 patients were free of malignancy. Of the 25 patients with adenocarcinoma, EGFR mutations were detected in 2 (8%) fresh tumor samples and in 3 (12%) fixed cytological smears. K-ras mutations were detected in 8 (32%) fresh samples, and in 9 (36%) fixed cytological smears. Fixed and stained cytological samples seem to be more reliable than fresh material for molecular analysis.

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July 2012
Volume 41 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ulivi P, Romagnoli M, Chiadini E, Casoni G, Capelli L, Gurioli C, Zoli W, Saragoni L, Dubini A, Tesei A, Tesei A, et al: Assessment of EGFR and K-ras mutations in fixed and fresh specimens from transesophageal ultrasound-guided fine needle aspiration in non-small cell lung cancer patients. Int J Oncol 41: 147-152, 2012
APA
Ulivi, P., Romagnoli, M., Chiadini, E., Casoni, G., Capelli, L., Gurioli, C. ... Poletti, V. (2012). Assessment of EGFR and K-ras mutations in fixed and fresh specimens from transesophageal ultrasound-guided fine needle aspiration in non-small cell lung cancer patients. International Journal of Oncology, 41, 147-152. https://doi.org/10.3892/ijo.2012.1432
MLA
Ulivi, P., Romagnoli, M., Chiadini, E., Casoni, G., Capelli, L., Gurioli, C., Zoli, W., Saragoni, L., Dubini, A., Tesei, A., Amadori, D., Poletti, V."Assessment of EGFR and K-ras mutations in fixed and fresh specimens from transesophageal ultrasound-guided fine needle aspiration in non-small cell lung cancer patients". International Journal of Oncology 41.1 (2012): 147-152.
Chicago
Ulivi, P., Romagnoli, M., Chiadini, E., Casoni, G., Capelli, L., Gurioli, C., Zoli, W., Saragoni, L., Dubini, A., Tesei, A., Amadori, D., Poletti, V."Assessment of EGFR and K-ras mutations in fixed and fresh specimens from transesophageal ultrasound-guided fine needle aspiration in non-small cell lung cancer patients". International Journal of Oncology 41, no. 1 (2012): 147-152. https://doi.org/10.3892/ijo.2012.1432