Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition

  • Authors:
    • Ursula L. Triantafillu
    • Seungjo Park
    • Nikki L. Klaassen
    • Andrew D. Raddatz
    • Yonghyun Kim
  • View Affiliations

  • Published online on: January 30, 2017     https://doi.org/10.3892/ijo.2017.3865
  • Pages:993-1001
Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Metastasis is the leading cause of cancer deaths due to the spread of cancer cells through the blood vessels and the subsequent formation of secondary tumors. Metastasizing cancer cells in the human vasculature are called circulating tumor cells (CTCs) and are characterized to express the epithelial cell adhesion molecule (EpCAM). They are further known to survive physiological fluid shear stress (FSS) conditions. However, the effect of FSS on CTC molecular phenotype, such as the epithelial to mesenchymal transition (EMT) and cancer stem cell (CSC) expression, has not been extensively studied. Here, CTCs in FSS are evaluated in an in vitro model system. MCF7 and MDA-MB-231 breast cancer cell lines were grown in adherent and suspension culture media. The cell lines were tested for EMT and CSC genetic and protein markers using qRT-PCR and flow cytometry, respectively. Suspension cells showed a significantly increased EMT signature compared to adherent cells (p<0.05), suggesting that they model cells detaching from primary tumors in vivo. Upon application of FSS, MCF7 and MDA-MB-231 cells did not show a significant change in EMT expression (p>0.05), but there was a statistically significant increase of the CSC population in MCF7 suspension cultures (p<0.05). These results with MCF7 suggest that CTCs can be modeled in vitro as non-adherent cancer cells in FSS and that they show an increased CSC-like signature during circulation, providing new insights to the importance of CSC-targeting strategies when treating metastatic patients.

Related Articles

Journal Cover

March 2017
Volume 50 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Triantafillu, U.L., Park, S., Klaassen, N.L., Raddatz, A.D., & Kim, Y. (2017). Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition. International Journal of Oncology, 50, 993-1001. https://doi.org/10.3892/ijo.2017.3865
MLA
Triantafillu, U. L., Park, S., Klaassen, N. L., Raddatz, A. D., Kim, Y."Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition". International Journal of Oncology 50.3 (2017): 993-1001.
Chicago
Triantafillu, U. L., Park, S., Klaassen, N. L., Raddatz, A. D., Kim, Y."Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition". International Journal of Oncology 50, no. 3 (2017): 993-1001. https://doi.org/10.3892/ijo.2017.3865