[Corrigendum] The pterocarpanquinone LQB-118 induces apoptosis in acute myeloid leukemia cells of distinct molecular subtypes and targets FoxO3a and FoxM1 transcription factors

• Authors:
  • Gabriela Nestal De Moraes
  • Carolina Pereira Castro
  • Eduardo Jesus Salustiano
  • Matheus Lourenço Dumas
  • Fernanda Costas
  • Eric Wing-Fai Lam
  • Paulo Roberto Ribeiro Costa
  • Raquel Ciuvalschi Maia

• View Affiliations

  
  Affiliations: Laboratory of Cellular and Molecular Hemato-Oncology, Program of Molecular Hemato-Oncology, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil, Laboratory of Bioorganic Chemistry, Research Nucleous of Natural Products, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil, Laboratory of Tumor Immunology, Leopoldo de Meis Medical Biochemistry Institute (IBqM-UFRJ), Rio de Janeiro, RJ, Brazil, Division of Cancer, Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London, UK
• Published online on: September 9, 2019     https://doi.org/10.3892/ijo.2019.4874
• Pages: 1396-1396

• Copyright : © Nestal De Moraes et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

Article

Int J Oncol 45: [Related article:] 1949-1958, 2014; DOI: 10.3892/ijo.2014.2615

Subsequent to the publication of the above article, the authors have realized that there were errors associated with Figs. 1c and 2b. In Fig. 1c, the authors noted that the same data were incorrectly presented for the 'Untreated cells" and 'DMSO' dot-blot experiments. After having re-examined their source data, the authors were able to confirm that the data correctly shown for the 'Untreated cells' experiment had inadvertently been included in the Figure as the data for the 'DMSO' experiment. Additionally, in Fig. 2b, the authors noticed that the percentage of untreated cells with active caspase-3 was missing (the label for the 'No antibody' experiment).

Figure 1 Flow cytometry analysis of LQB-118-induced cell death in HL60 and U937 AML cell lines. HL60 and U937 cell lines were exposed to 1.5, 3, 6 and 9 μM of LQB-118 and to dimethyl sulfoxide (DMSO) and collected after a 24-h exposure. (a and b) DNA fragmentation was evaluated in the cell lines and estimated by the percentage of cells in the sub-G0/G1 phase. (c and d) Cells were stained with Annexin V and propidium iodide for detection of Annexin V positivity. DMSO was used as a vector control for the highest concentration. The images are representative of three independent experiments.

Figure 2 Profile of caspase-3 activation mediated by the LQB-118 compound in HL60 and U937 cells. (a) HL60 and (b) U937 cell lines were incubated with 1.5, 3, 6 and 9 μM LQB-118 and with dimethyl sulfoxide (DMSO). After 24 h of incubation, cells were harvested and analyzed for caspase-3 activation using flow cytometry, as described in the Materials and methods. The percentages of LQB-118-induced caspase-3 activation were calculated by subtracting the values of the caspase-3 staining from the negative control samples (no antibody). The histograms are representative of three independent experiments.

Corrected versions of Figs. 1 (including the correct data for the 'DMSO' dot blot) and 2 (with the label now incorporated) are shown opposite. Note that these changes do not affect the results or the conclusions reported in this paper, and all the authors agree to this correction. The authors apologize to the Editor and to the readership of the Journal for any inconvenience caused.