Open Access

ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

  • Authors:
    • Xiao‑Fang Liu
    • Qiang Han
    • Xue‑Zhu Rong
    • Man Yang
    • Yu‑Chen Han
    • Juan‑Han Yu
    • Xu‑Yong Lin
  • View Affiliations

  • Published online on: February 19, 2020     https://doi.org/10.3892/ijo.2020.4994
  • Pages: 1175-1185
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The ankyrin repeat and KH domain‑containing 1 (ANKHD1) protein was recently reported to be a potential member of the Hippo signaling pathway. However, its role in human non‑small‑cell lung cancer (NSCLC) has not been extensively investigated. The aim of the present study was to examine the expression of ANKHD1 in primary human tissues and cells and determine whether it is correlated with the clinical characteristics of tumor growth. The biological functions of ANKHD1 were evaluated in vitro and in vivo. Yes‑associated protein (YAP) expression and phosphorylation induced by ANKHD1 were evaluated by western blotting and immunoprecipitation. Marked upregulation of ANKHD1 protein expression was observed in NSCLC cells and tissues, which was associated with advanced pathological tumor‑node‑metastasis stage, lymph node metastasis and poor prognosis in patients with NSCLC. ANKHD1 overexpression also promoted the proliferation and invasion of NSCLC cells. ANKHD1 upregulation inactivated Hippo signaling via increasing YAP protein levels, as well as inhibiting YAP protein phosphorylation, whereas depletion of YAP abolished the effects of ANKHD1 on cell proliferation and invasion. Therefore, ANKHD1 may play an important role in NSCLC through regulating the YAP‑dependent Hippo signaling pathway.

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May 2020
Volume 56 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Liu, X., Han, Q., Rong, X., Yang, M., Han, Y., Yu, J., & Lin, X. (2020). ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. International Journal of Oncology, 56, 1175-1185. https://doi.org/10.3892/ijo.2020.4994
MLA
Liu, X., Han, Q., Rong, X., Yang, M., Han, Y., Yu, J., Lin, X."ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway". International Journal of Oncology 56.5 (2020): 1175-1185.
Chicago
Liu, X., Han, Q., Rong, X., Yang, M., Han, Y., Yu, J., Lin, X."ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway". International Journal of Oncology 56, no. 5 (2020): 1175-1185. https://doi.org/10.3892/ijo.2020.4994