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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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July 2002 Volume 21 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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July 2002 Volume 21 Issue 1

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Article

Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor

  • Authors:
    • J. Nishio
    • H. Iwasaki
    • M. Ishiguro
    • Y. Ohjimi
    • C. Fujita
    • T. Isayama
    • M. Naito
    • Y. Oda
    • Y. Kaneko
    • M. Kikuchi
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan. nishio@minf.med.fukuoka-u.ac.jp
  • Pages: 17-23
    |
    Published online on: July 1, 2002
       https://doi.org/10.3892/ijo.21.1.17
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Abstract

Only a small number of human synovial sarcoma cell lines have been reported, and of those, not all have been fully characterized, especially at the molecular level. We describe here the establishment and characterization of a new human cell line, FU-SY-1, which originated from a monophasic fibrous synovial sarcoma arising in the supinator muscle of a 31-year-old woman. This cell line propagated continuously in vitro for 73 serial passages for more than 36 months. FU-SY-1 cells in vitro were rather small, exhibited a spindle or polygonal shape without conspicuous pleomorphism, and expressed c-Met and hepatocyte growth factor (HGF) as determined by immunocytochemistry. Cytogenetically, FU-SY-1 cells maintained a consistent karyotype: 47, X, +7, t(X;18)(p11.2;q11.2), the same as that of the original tumor specimen. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated a SYT-SSX fusion transcript and expression of c-Met and HGF mRNA in FU-SY-1 cells. A subsequent sequence analysis using the PCR products confirmed that the detected messages were derived from the SYT-SSX1 fusion gene. This cell line, FU-SY-1, established from a monophasic fibrous synovial sarcoma, may therefore be a useful tool for investigation of the mechanisms of tumorigenesis and progression in human synovial sarcomas.

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Copy and paste a formatted citation
Spandidos Publications style
Nishio J, Iwasaki H, Ishiguro M, Ohjimi Y, Fujita C, Isayama T, Naito M, Oda Y, Kaneko Y, Kikuchi M, Kikuchi M, et al: Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor. Int J Oncol 21: 17-23, 2002.
APA
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T. ... Kikuchi, M. (2002). Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor. International Journal of Oncology, 21, 17-23. https://doi.org/10.3892/ijo.21.1.17
MLA
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T., Naito, M., Oda, Y., Kaneko, Y., Kikuchi, M."Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor". International Journal of Oncology 21.1 (2002): 17-23.
Chicago
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T., Naito, M., Oda, Y., Kaneko, Y., Kikuchi, M."Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor". International Journal of Oncology 21, no. 1 (2002): 17-23. https://doi.org/10.3892/ijo.21.1.17
Copy and paste a formatted citation
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Spandidos Publications style
Nishio J, Iwasaki H, Ishiguro M, Ohjimi Y, Fujita C, Isayama T, Naito M, Oda Y, Kaneko Y, Kikuchi M, Kikuchi M, et al: Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor. Int J Oncol 21: 17-23, 2002.
APA
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T. ... Kikuchi, M. (2002). Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor. International Journal of Oncology, 21, 17-23. https://doi.org/10.3892/ijo.21.1.17
MLA
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T., Naito, M., Oda, Y., Kaneko, Y., Kikuchi, M."Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor". International Journal of Oncology 21.1 (2002): 17-23.
Chicago
Nishio, J., Iwasaki, H., Ishiguro, M., Ohjimi, Y., Fujita, C., Isayama, T., Naito, M., Oda, Y., Kaneko, Y., Kikuchi, M."Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor". International Journal of Oncology 21, no. 1 (2002): 17-23. https://doi.org/10.3892/ijo.21.1.17
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