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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August 2002 Volume 21 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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August 2002 Volume 21 Issue 2

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Article

Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth

  • Authors:
    • Seiichiro Sakao
    • Tomohiro Hashimoto
    • Yuji Shino
    • Takeshi Saito
    • Satoshi Kado
    • Hiroshi Sashiyama
    • Koichiro Tatsumi
    • Takayuki Kuriyama
    • Hiroshi Shirasawa
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Virology, Chiba University School of Medicine, Chiba 260-8670, Japan
  • Pages: 273-279
    |
    Published online on: August 1, 2002
       https://doi.org/10.3892/ijo.21.2.273
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Abstract

A novel gene transcript that is downregulated by HPV16 E6 protein was identified in mouse cells using differential hybridization and designated E6DG1. The cloned cDNA of E6DG1 was 1.3 kb in length and contained a small ORF potentially encoding a polypeptide of 45 amino acids. In vitro transcription and translation of E6DG1 cDNA resulted in a product of approximately 7 kDa and Western blot analysis using antibodies for E6DG1 peptide detected 7 and 14 kDa proteins. Downregulation of E6DG1 mRNA levels in cells expressing HPV16 E6 protein was observed at subconfluent cell densities, but not in confluent cells. Repression of E6DG1 protein enhanced the anchorage-independent growth and weakened the cell adhesion in Panc1 cells. Immunofluorescence analysis revealed the localization of E6DG1 within the nucleus. These results indicate that the E6DG1 protein may function in a signaling pathway related to anchorage-independent growth and adhesion control.

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Copy and paste a formatted citation
Spandidos Publications style
Sakao S, Hashimoto T, Shino Y, Saito T, Kado S, Sashiyama H, Tatsumi K, Kuriyama T and Shirasawa H: Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. Int J Oncol 21: 273-279, 2002.
APA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H. ... Shirasawa, H. (2002). Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. International Journal of Oncology, 21, 273-279. https://doi.org/10.3892/ijo.21.2.273
MLA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21.2 (2002): 273-279.
Chicago
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21, no. 2 (2002): 273-279. https://doi.org/10.3892/ijo.21.2.273
Copy and paste a formatted citation
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Spandidos Publications style
Sakao S, Hashimoto T, Shino Y, Saito T, Kado S, Sashiyama H, Tatsumi K, Kuriyama T and Shirasawa H: Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. Int J Oncol 21: 273-279, 2002.
APA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H. ... Shirasawa, H. (2002). Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. International Journal of Oncology, 21, 273-279. https://doi.org/10.3892/ijo.21.2.273
MLA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21.2 (2002): 273-279.
Chicago
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21, no. 2 (2002): 273-279. https://doi.org/10.3892/ijo.21.2.273
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