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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August 2002 Volume 21 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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August 2002 Volume 21 Issue 2

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Article

DNA content and cell number determination in microdissected samples of breast carcinoma in situ

  • Authors:
    • Robert Blumenstein
    • Margarida Dias
    • Irma H. Russo
    • Quivo Tahin
    • Jose Russo

  • View Affiliations / Copyright

    Affiliations: DeSales University, Center Valley, PA, USA
  • Pages: 447-450
    |
    Published online on: August 1, 2002
       https://doi.org/10.3892/ijo.21.2.447
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Abstract

Paraffin-embedded tissue (PET) is the specimen of choice for the histopathological diagnosis of cancer. PET has become a valuable resource for correlating cellular phenotype and genotype in microdissected lesions. A definitive improvement in this field has been the development of infra-red laser capture microdissection (LCM), which yields homogeneous populations of cells for DNA extraction and in vitro amplification by polymerase chain reaction (PCR). We report here a photographic and fluorescent technique for determining the number of nuclei and concentration of DNA obtained respectively by laser capture microdissection from paraffin-embedded breast cancer tissue. Breast biopsies containing carcinoma in situ were serially sectioned, mounted on plain glass slides and tumor cells were microdissected using a laser capture microscope. The DNA was extracted in digestion buffer and used directly as a template for PCR. In our protocols, each capture contained 21±5.4 nuclei with a DNA concentration of 115±5.3 pg. Also, a linear relationship was found between number of captures and DNA content (R2=0.9995). These results represent a novel contribution for a more precise correlation between phenotypic and genotypic diversity in cancer cells studied from microdissected paraffin-embedded tissue.

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Copy and paste a formatted citation
Spandidos Publications style
Blumenstein R, Dias M, Russo IH, Tahin Q and Russo J: DNA content and cell number determination in microdissected samples of breast carcinoma in situ. Int J Oncol 21: 447-450, 2002.
APA
Blumenstein, R., Dias, M., Russo, I.H., Tahin, Q., & Russo, J. (2002). DNA content and cell number determination in microdissected samples of breast carcinoma in situ. International Journal of Oncology, 21, 447-450. https://doi.org/10.3892/ijo.21.2.447
MLA
Blumenstein, R., Dias, M., Russo, I. H., Tahin, Q., Russo, J."DNA content and cell number determination in microdissected samples of breast carcinoma in situ". International Journal of Oncology 21.2 (2002): 447-450.
Chicago
Blumenstein, R., Dias, M., Russo, I. H., Tahin, Q., Russo, J."DNA content and cell number determination in microdissected samples of breast carcinoma in situ". International Journal of Oncology 21, no. 2 (2002): 447-450. https://doi.org/10.3892/ijo.21.2.447
Copy and paste a formatted citation
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Spandidos Publications style
Blumenstein R, Dias M, Russo IH, Tahin Q and Russo J: DNA content and cell number determination in microdissected samples of breast carcinoma in situ. Int J Oncol 21: 447-450, 2002.
APA
Blumenstein, R., Dias, M., Russo, I.H., Tahin, Q., & Russo, J. (2002). DNA content and cell number determination in microdissected samples of breast carcinoma in situ. International Journal of Oncology, 21, 447-450. https://doi.org/10.3892/ijo.21.2.447
MLA
Blumenstein, R., Dias, M., Russo, I. H., Tahin, Q., Russo, J."DNA content and cell number determination in microdissected samples of breast carcinoma in situ". International Journal of Oncology 21.2 (2002): 447-450.
Chicago
Blumenstein, R., Dias, M., Russo, I. H., Tahin, Q., Russo, J."DNA content and cell number determination in microdissected samples of breast carcinoma in situ". International Journal of Oncology 21, no. 2 (2002): 447-450. https://doi.org/10.3892/ijo.21.2.447
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