Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines

  • Authors:
    • Keiichiro Nakamura
    • Yutaka Yasunaga
    • Takehiko Segawa
    • Daejin Ko
    • Judd W. Moul
    • Shiv Srivastava
    • Johng S. Rhim
  • View Affiliations

  • Published online on: Tuesday, October 1, 2002
  • Pages: 825-830 DOI: 10.3892/ijo.21.4.825

Abstract

Curcumin, traditionally used as a seasoning spice in Indian cuisine, has been reported to decrease the proliferation potential of prostate cancer cells, by a mechanism that is not fully understood. In the current study, we have evaluated the effects of curcumin in cell growth, activation of signal transduction, and transforming activities of both androgen-dependent and independent cell lines. Prostate cancer cell lines, LNCaP and PC-3, were treated with curcumin and its effects were further analyzed on signal transduction and expression of androgen receptor (AR) and AR-related cofactors using transient transfection assay and Western blotting. Our results show that curcumin down-regulates transactivation and expression of AR, activator protein-1 (AP-1), nuclear factor-κB (NF-κB), and CREB (cAMP response element-binding protein)-binding protein (CBP). Curcumin also inhibited the transforming activities of both cell lines as evidenced by the reduced colony forming ability in soft agar. The results obtained here demonstrate that curcumin has a potential therapeutic effect on prostate cancer cells through down-regulation of AR and AR-related cofactors (AP-1, NF-κB and CBP).

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October 2002
Volume 21 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
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APA
Nakamura, K., Yasunaga, Y., Segawa, T., Ko, D., Moul, J.W., Srivastava, S., & Rhim, J.S. (2002). Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. International Journal of Oncology, 21, 825-830. http://dx.doi.org/10.3892/ijo.21.4.825
MLA
Nakamura, K., Yasunaga, Y., Segawa, T., Ko, D., Moul, J. W., Srivastava, S., Rhim, J. S."Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines". International Journal of Oncology 21.4 (2002): 825-830.
Chicago
Nakamura, K., Yasunaga, Y., Segawa, T., Ko, D., Moul, J. W., Srivastava, S., Rhim, J. S."Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines". International Journal of Oncology 21, no. 4 (2002): 825-830. http://dx.doi.org/10.3892/ijo.21.4.825