Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line

  • Authors:
    • Tetsuji Tokunaga
    • Yoshiyuki Abe
    • Takashi Tsuchida
    • Hiroyuki Hatanaka
    • Yoshiro Oshika
    • Masashi Tomisawa
    • Masumi Yoshimura
    • Yasuyuki Ohnishi
    • Hiroshi Kijima
    • Hitoshi Yamazaki
    • Yoshito Ueyama
    • Masato Nakamura
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijo.21.5.1027
  • Pages: 1027-1032
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Abstract

Stromal angiogenesis is an important factor for progression of malignant neoplasms. We used hammerhead ribozymes against vascular endothelial growth factor (VEGF) gene transcripts to down-regulate cell-associated VEGF189 isoform function in the pancreatic cancer cell line MIA PaCa2. MIA PaCa2 transfected with anti-VEGF189 ribozyme did not show any alteration of growth rate under tissue culture. When the transformants were subcutaneously transplanted, tumour volume of the ribozyme-transfected MIA PaCa2 xenografts was significantly smaller (P<0.01). No metastasis of MIA PaCa2 transfected with anti-VEGF189 was apparent, while disabled ribozyme-transfected MIA PaCa2 showed significant liver metastasis (P<0.05). These results suggested that VEGF189 plays an important role in growth and metastatic potential through alteration of angiogenic balance in cancer.

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November 2002
Volume 21 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tokunaga T, Abe Y, Tsuchida T, Hatanaka H, Oshika Y, Tomisawa M, Yoshimura M, Ohnishi Y, Kijima H, Yamazaki H, Yamazaki H, et al: Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line. Int J Oncol 21: 1027-1032, 2002
APA
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M. ... Nakamura, M. (2002). Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line. International Journal of Oncology, 21, 1027-1032. https://doi.org/10.3892/ijo.21.5.1027
MLA
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M., Yoshimura, M., Ohnishi, Y., Kijima, H., Yamazaki, H., Ueyama, Y., Nakamura, M."Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line". International Journal of Oncology 21.5 (2002): 1027-1032.
Chicago
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M., Yoshimura, M., Ohnishi, Y., Kijima, H., Yamazaki, H., Ueyama, Y., Nakamura, M."Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line". International Journal of Oncology 21, no. 5 (2002): 1027-1032. https://doi.org/10.3892/ijo.21.5.1027