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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2002 Volume 21 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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November 2002 Volume 21 Issue 5

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Article

Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells

  • Authors:
    • Emmanuelle Charafe-Jauffret
    • Jean-Francois Moulin
    • Christophe Ginestier
    • Didier Bechlian
    • Nathalie Conte
    • Jeannine Geneix
    • Jose Adelaide
    • Tetsuro Noguchi
    • Jacques Hassoun
    • Jocelyne Jacquemier
    • Daniel Birnbaum
  • View Affiliations / Copyright

    Affiliations: Departement d'Oncologie Moleculaire, Institut Paoli-Calmettes (IPC), Marseille, France
  • Pages: 989-996
    |
    Published online on: November 1, 2002
       https://doi.org/10.3892/ijo.21.5.989
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Abstract

Alterations of chromosomal region 8p11-21 are very frequent in human cancers, and especially in breast cancer; yet, most of the genes involved have not been identified. We performed laser capture microdissection in a series of 52 consecutive breast tumor samples to obtain pure tumor cells without surrounding normal breast. To determine genomic subregions in which some of the cancer genes may be located, we conducted a search for loss of heterozygosity (LOH) at 13 microsatellite markers from this region. Two-thirds of the tumors showed LOH at least at one marker. Microdissection of pure tumor samples was helpful to precisely define four LOH subregions. No LOH was observed in the corresponding peritumoral tissues. We studied by immunohistochemistry (IHC) on tissue-microarrays the expression in the same tumors, of the protein product of three potential tumor genes lying close to or within the subregions of LOH. In most samples, the TACC1 gene product was downregulated in tumor cells as compared to normal cells. Our results show that the centromeric portion of chromosome arm 8p is frequently altered in breast tumor cells.

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Copy and paste a formatted citation
Spandidos Publications style
Charafe-Jauffret E, Moulin J, Ginestier C, Bechlian D, Conte N, Geneix J, Adelaide J, Noguchi T, Hassoun J, Jacquemier J, Jacquemier J, et al: Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells. Int J Oncol 21: 989-996, 2002.
APA
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J. ... Birnbaum, D. (2002). Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells. International Journal of Oncology, 21, 989-996. https://doi.org/10.3892/ijo.21.5.989
MLA
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J., Adelaide, J., Noguchi, T., Hassoun, J., Jacquemier, J., Birnbaum, D."Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells". International Journal of Oncology 21.5 (2002): 989-996.
Chicago
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J., Adelaide, J., Noguchi, T., Hassoun, J., Jacquemier, J., Birnbaum, D."Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells". International Journal of Oncology 21, no. 5 (2002): 989-996. https://doi.org/10.3892/ijo.21.5.989
Copy and paste a formatted citation
x
Spandidos Publications style
Charafe-Jauffret E, Moulin J, Ginestier C, Bechlian D, Conte N, Geneix J, Adelaide J, Noguchi T, Hassoun J, Jacquemier J, Jacquemier J, et al: Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells. Int J Oncol 21: 989-996, 2002.
APA
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J. ... Birnbaum, D. (2002). Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells. International Journal of Oncology, 21, 989-996. https://doi.org/10.3892/ijo.21.5.989
MLA
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J., Adelaide, J., Noguchi, T., Hassoun, J., Jacquemier, J., Birnbaum, D."Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells". International Journal of Oncology 21.5 (2002): 989-996.
Chicago
Charafe-Jauffret, E., Moulin, J., Ginestier, C., Bechlian, D., Conte, N., Geneix, J., Adelaide, J., Noguchi, T., Hassoun, J., Jacquemier, J., Birnbaum, D."Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells". International Journal of Oncology 21, no. 5 (2002): 989-996. https://doi.org/10.3892/ijo.21.5.989
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