Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis

  • Authors:
    • Woo Hyun Park
    • Chul Won Jung
    • Joon Oh Park
    • Kihyun Kim
    • Won Seog Kim
    • Young Hyuck Im
    • Mark H. Lee
    • Won Ki Kang
    • Keunchil Park
  • View Affiliations

  • Published online on: April 1, 2003     https://doi.org/10.3892/ijo.22.4.855
  • Pages: 855-860
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Abstract

Previously, we showed that monensin, Na+ ionophore, potently inhibited the growth of acute myelogenous leukemia and lymphoma cells. Here, we demonstrate that monensin inhibited the proliferation of renal cell carcinoma cells with IC50 of about 2.5 µM. Monensin induced a G1 or a G2-M phase arrest in these cells. When we examined the effects of this drug on ACHN cells, monensin decreased the levels of CDK2, CDK6, cdc2, cyclin A and cyclin B1 proteins. p21 and p27 proteins were increased by monensin. In addition, monensin markedly enhanced the binding of p21 with CDK2 and the binding of p27 with CDK6. Furthermore, the activities of CDK2- and CDK6-associated kinase were reduced in association with hypophosphorylation of Rb protein. Monensin also induced the apoptosis in several renal cell carcinoma cells. Apoptotic process of Caki-2 cells was associated with the changes of Bcl-2, Bcl-XL, caspase-9, caspase-3, caspase-7 proteins as well as mitochondria transmembrane potential (ΔΨm) loss. Taken together, these results demonstrate for the first time that monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis.

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April 2003
Volume 22 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park WH, Jung CW, Park JO, Kim K, Kim WS, Im YH, Lee MH, Kang WK and Park K: Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis. Int J Oncol 22: 855-860, 2003.
APA
Park, W.H., Jung, C.W., Park, J.O., Kim, K., Kim, W.S., Im, Y.H. ... Park, K. (2003). Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis. International Journal of Oncology, 22, 855-860. https://doi.org/10.3892/ijo.22.4.855
MLA
Park, W. H., Jung, C. W., Park, J. O., Kim, K., Kim, W. S., Im, Y. H., Lee, M. H., Kang, W. K., Park, K."Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis". International Journal of Oncology 22.4 (2003): 855-860.
Chicago
Park, W. H., Jung, C. W., Park, J. O., Kim, K., Kim, W. S., Im, Y. H., Lee, M. H., Kang, W. K., Park, K."Monensin inhibits the growth of renal cell carcinoma cells via cell cycle arrest or apoptosis". International Journal of Oncology 22, no. 4 (2003): 855-860. https://doi.org/10.3892/ijo.22.4.855