Mutation analysis of the BRG1 gene in prostate cancer clinical samples

  • Authors:
    • Alexander Valdman
    • Agneta Nordenskjöld
    • Xiaolei Fang
    • Ayako Naito
    • Salman Al-Shukri
    • Catharina Larsson
    • Peter Ekman
    • Chunde Li
  • View Affiliations

  • Published online on: May 1, 2003     https://doi.org/10.3892/ijo.22.5.1003
  • Pages: 1003-1007
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Abstract

Previous studies in hereditary and sporadic prostate cancer have indicated the existence of a tumor suppressor gene in chromosomal region 19p13. The BRG1 gene in this region is one of the possible candidates, based on both the frequency of inactivating mutations in human cancer cell lines, including the prostate cancer cell line DU145, and its functional properties. To our knowledge, no studies have been done to evaluate possible involvement of the BRG1 gene in clinical prostate cancer. To accomplish this, we carried out a complete mutation analysis of all 35 BRG1 exons in tumor and constitutional DNA samples from 21 prostate cancer patients. We report the absence of somatic mutations in the panel of samples employed, but the existence of five germline single nucleotide polymorphisms (SNPs) in CpG islands of the BRG1 gene, among them, three novel ones. In conclusion, the study excludes the presence of common BRG1 mutations in prostate cancer.

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May 2003
Volume 22 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Valdman A, Nordenskjöld A, Fang X, Naito A, Al-Shukri S, Larsson C, Ekman P and Li C: Mutation analysis of the BRG1 gene in prostate cancer clinical samples. Int J Oncol 22: 1003-1007, 2003
APA
Valdman, A., Nordenskjöld, A., Fang, X., Naito, A., Al-Shukri, S., Larsson, C. ... Li, C. (2003). Mutation analysis of the BRG1 gene in prostate cancer clinical samples. International Journal of Oncology, 22, 1003-1007. https://doi.org/10.3892/ijo.22.5.1003
MLA
Valdman, A., Nordenskjöld, A., Fang, X., Naito, A., Al-Shukri, S., Larsson, C., Ekman, P., Li, C."Mutation analysis of the BRG1 gene in prostate cancer clinical samples". International Journal of Oncology 22.5 (2003): 1003-1007.
Chicago
Valdman, A., Nordenskjöld, A., Fang, X., Naito, A., Al-Shukri, S., Larsson, C., Ekman, P., Li, C."Mutation analysis of the BRG1 gene in prostate cancer clinical samples". International Journal of Oncology 22, no. 5 (2003): 1003-1007. https://doi.org/10.3892/ijo.22.5.1003