Effects of overexpression of the Ets family transcription factor TEL on cell growth and differentiation of K562 cells

  • Authors:
    • Takuya Sakurai
    • Toshiyuki Yamada
    • Fumiko Kihara-Negishi
    • Sayaka Teramoto
    • Yuko Sato
    • Tetsuya Izawa
    • Tsuneyuki Oikawa
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijo.22.6.1327
  • Pages: 1327-1333
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Abstract

Functional roles of several Ets transcription factors in megakaryocytic and erythroid differentiation of the human chronic myelogenous leukemia cell line K562 was investigated. When K562 cells were induced to differentiate into the megakaryocyte lineage by treatment with TPA, the expression of the c-ets-1, Fli-1 and TEL2 genes was increased, and that of the TEL gene was decreased at the onset of differentiation. When the cells were induced to differentiate into the erythroid lineage by treatment with Hemin, expression of the c-ets-1 gene was increased, and that of the Fli-1 and TEL genes was decreased. To investigate the role of TEL in the growth and differentiation of K562 cells, we introduced an expression plasmid containing the TEL gene into the cells. Overexpression of TEL in K562 cells leads to inhibition of the endogenous expression of megakaryocyte-specific GPIIb and GPIbα genes, and inhibition of cell growth. DNA array analysis revealed that expression of genes such as the RhoG and D4-GDI genes was down-regulated in TEL-overexpressing cells, while that of the representative growth-related genes such as the c-myc, c-fos and c-jun genes was not remarkably changed. These results suggest that several Ets family transcription factors are implicated in megakaryocytic and erythroid differentiation of K562 cells and TEL inhibits the expression of some megakaryocyte-specific genes and growth in K562 cells.

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June 2003
Volume 22 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sakurai T, Yamada T, Kihara-Negishi F, Teramoto S, Sato Y, Izawa T and Oikawa T: Effects of overexpression of the Ets family transcription factor TEL on cell growth and differentiation of K562 cells. Int J Oncol 22: 1327-1333, 2003.
APA
Sakurai, T., Yamada, T., Kihara-Negishi, F., Teramoto, S., Sato, Y., Izawa, T., & Oikawa, T. (2003). Effects of overexpression of the Ets family transcription factor TEL on cell growth and differentiation of K562 cells. International Journal of Oncology, 22, 1327-1333. https://doi.org/10.3892/ijo.22.6.1327
MLA
Sakurai, T., Yamada, T., Kihara-Negishi, F., Teramoto, S., Sato, Y., Izawa, T., Oikawa, T."Effects of overexpression of the Ets family transcription factor TEL on cell growth and differentiation of K562 cells". International Journal of Oncology 22.6 (2003): 1327-1333.
Chicago
Sakurai, T., Yamada, T., Kihara-Negishi, F., Teramoto, S., Sato, Y., Izawa, T., Oikawa, T."Effects of overexpression of the Ets family transcription factor TEL on cell growth and differentiation of K562 cells". International Journal of Oncology 22, no. 6 (2003): 1327-1333. https://doi.org/10.3892/ijo.22.6.1327