Galactosyltransferase associated with tumor in patients with ovarian cancer: Factors involved in elevation of serum galactosyltransferase

  • Authors:
    • Eiko Saitoh
    • Daisuke Aoki
    • Nobuyuki Susumu
    • Yasuhiro Udagawa
    • Shiro Nozawa
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijo.23.2.303
  • Pages: 303-310
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Abstract

The serum level of β1,4-galactosyltransferase (β1,4-GalT) is increased in both malignancy and benign diseases. Galactosyltransferase associated with tumor (GAT) is one of the soluble forms of β1,4-GalT, and is a marker of ovarian cancer with a high specificity. GAT and normal soluble β1,4-GalT are both derived from the same membrane-bound form of the enzyme. This study investigated the mechanism of GAT elevation in patients with ovarian cancer. The serum levels of GAT and normal β1,4-GalT were measured using specific monoclonal antibodies. In addition, nude mice bearing human ovarian cancer were used to assess the kinetics of tumor-derived enzymes. GAT and normal β1,4-GalT were both detected in ovarian cancer patients, but only GAT reflected the tumor status. In tumor-bearing nude mice, both soluble forms of β1,4-GalT were released from tumor cells, but the half-life of GAT was far shorter than that of normal β1,4-GalT. Addition of serum from healthy women to colostrum (which has a high GAT content) reduced the GAT level, while adding patient serum caused a significantly smaller reduction of GAT. Addition of the serum from mouse which includes no human β1,4-GalT to colostrum also reduced the GAT level with no significant change of total soluble β1,4-GalT. These findings indicate that human serum contains certain factors that decrease the GAT level, but these factors are inhibited in ovarian cancer patients so that a high GAT level persists. It seems that the decrease of GAT occurs as a result of conversion into normal β1,4-GalT.

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August 2003
Volume 23 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Saitoh E, Aoki D, Susumu N, Udagawa Y and Nozawa S: Galactosyltransferase associated with tumor in patients with ovarian cancer: Factors involved in elevation of serum galactosyltransferase. Int J Oncol 23: 303-310, 2003
APA
Saitoh, E., Aoki, D., Susumu, N., Udagawa, Y., & Nozawa, S. (2003). Galactosyltransferase associated with tumor in patients with ovarian cancer: Factors involved in elevation of serum galactosyltransferase. International Journal of Oncology, 23, 303-310. https://doi.org/10.3892/ijo.23.2.303
MLA
Saitoh, E., Aoki, D., Susumu, N., Udagawa, Y., Nozawa, S."Galactosyltransferase associated with tumor in patients with ovarian cancer: Factors involved in elevation of serum galactosyltransferase". International Journal of Oncology 23.2 (2003): 303-310.
Chicago
Saitoh, E., Aoki, D., Susumu, N., Udagawa, Y., Nozawa, S."Galactosyltransferase associated with tumor in patients with ovarian cancer: Factors involved in elevation of serum galactosyltransferase". International Journal of Oncology 23, no. 2 (2003): 303-310. https://doi.org/10.3892/ijo.23.2.303