Differentially expressed genes and estrogen receptor status in breast cancer

  • Authors:
    • Maria Aparecida Nagai
    • Nancy Ros
    • Simone Aparecida Bessa
    • Mário Mourão Neto
    • Elisabete Cristina Miracca
    • Maria Mitzi Brentani
  • View Affiliations

  • Published online on: November 1, 2003     https://doi.org/10.3892/ijo.23.5.1425
  • Pages: 1425-1430
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Abstract

There is a large and increasing body of experimental and clinical data supporting the involvement of estrogen on the proliferation of hormone-dependent breast tumors. Estrogen acts via its receptor (ER) stimulating cellular proliferation. ER and progesterone receptor (PR), which is regulated by estrogen via ER, have been used as prognostic markers in the clinical management of breast cancer patients. The aim of the present study was the identification of tumor-associated genes differentially expressed in breast tumors regarding the presence or absence of ER and PR. Using the technique of differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) we have isolated and cloned 127 cDNA fragments that showed differential expression in either ER+/PR+ or ER−/PR− breast tumors. Sequencing analysis of these clones revealed that 119 cDNAs had homology with known sequences in the National Center of Biotechnology Information (NCBI) and 8 were novel, showing no homology to known genes. Among these differentially expressed transcripts are metabolic enzymes, ribosomal proteins, transcription factors, hypothetical proteins, cell cycle regulators, cytoskelectum related genes, cell adhesion and motility genes. Differences in gene expression profiles are likely to explain the phenotypic differences between hormone-responsive and hormone-unresponsive breast tumors.

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November 2003
Volume 23 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nagai MA, Ros N, Bessa SA, Neto MM, Miracca EC and Brentani MM: Differentially expressed genes and estrogen receptor status in breast cancer. Int J Oncol 23: 1425-1430, 2003
APA
Nagai, M.A., Ros, N., Bessa, S.A., Neto, M.M., Miracca, E.C., & Brentani, M.M. (2003). Differentially expressed genes and estrogen receptor status in breast cancer. International Journal of Oncology, 23, 1425-1430. https://doi.org/10.3892/ijo.23.5.1425
MLA
Nagai, M. A., Ros, N., Bessa, S. A., Neto, M. M., Miracca, E. C., Brentani, M. M."Differentially expressed genes and estrogen receptor status in breast cancer". International Journal of Oncology 23.5 (2003): 1425-1430.
Chicago
Nagai, M. A., Ros, N., Bessa, S. A., Neto, M. M., Miracca, E. C., Brentani, M. M."Differentially expressed genes and estrogen receptor status in breast cancer". International Journal of Oncology 23, no. 5 (2003): 1425-1430. https://doi.org/10.3892/ijo.23.5.1425