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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2003 Volume 23 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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November 2003 Volume 23 Issue 5

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Article

Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin

  • Authors:
    • Appolinary A.R. Kamuhabwa
    • Ann Huygens
    • Peter A.M. De Witte

  • View Affiliations / Copyright

    Affiliations: Laboratorium voor Farmaceutische Biologie en Fytofarmacologie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium
  • Pages: 1445-1450
    |
    Published online on: November 1, 2003
       https://doi.org/10.3892/ijo.23.5.1445
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Abstract

The objective of the present study was to evaluate the sensitivity of the bladder transitional cell carcinoma (TCC) to hypericin PDT in a 3-D system using multicellular tumor spheroids. The photodynamic response in RT-112 human bladder TCC spheroids was also compared to 2-D cultured monolayer cells. Following a 2-4 h incubation with 8-30 μM hypericin, spheroids or monolayer cells were irradiated at the light dose of 12 J/cm2, delivered at a fluence rate of 10-100 mW/cm2. The PDT effects were evaluated using a clonogenic assay. The results show that compared with the cells in a monolayer, cells in spheroids were dramatically less sensitive to hypericin PDT (<2000-fold). Studies of fluorescence microphotographs of centrally cut frozen sections of hypericin-exposed spheroids showed a gradient in hypericin concentration from the peripheral to the central region of the spheroid. Although it can be suggested that heterogeneity of drug uptake might be responsible for the observed resistance of spheroid to hypericin PDT, hypericin sensitized spheroids that were dissociated prior to light irradiation were as sensitive as the monolayer cells to hypericin PDT, suggesting that other factors such as oxygen depletion might be responsible for the resistance of spheroids to hypericin PDT.

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Copy and paste a formatted citation
Spandidos Publications style
Kamuhabwa AA, Huygens A and De Witte PA: Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin. Int J Oncol 23: 1445-1450, 2003.
APA
Kamuhabwa, A.A., Huygens, A., & De Witte, P.A. (2003). Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin. International Journal of Oncology, 23, 1445-1450. https://doi.org/10.3892/ijo.23.5.1445
MLA
Kamuhabwa, A. A., Huygens, A., De Witte, P. A."Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin". International Journal of Oncology 23.5 (2003): 1445-1450.
Chicago
Kamuhabwa, A. A., Huygens, A., De Witte, P. A."Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin". International Journal of Oncology 23, no. 5 (2003): 1445-1450. https://doi.org/10.3892/ijo.23.5.1445
Copy and paste a formatted citation
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Spandidos Publications style
Kamuhabwa AA, Huygens A and De Witte PA: Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin. Int J Oncol 23: 1445-1450, 2003.
APA
Kamuhabwa, A.A., Huygens, A., & De Witte, P.A. (2003). Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin. International Journal of Oncology, 23, 1445-1450. https://doi.org/10.3892/ijo.23.5.1445
MLA
Kamuhabwa, A. A., Huygens, A., De Witte, P. A."Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin". International Journal of Oncology 23.5 (2003): 1445-1450.
Chicago
Kamuhabwa, A. A., Huygens, A., De Witte, P. A."Photodynamic therapy of transitional cell carcinoma multicellular tumor spheroids with hypericin". International Journal of Oncology 23, no. 5 (2003): 1445-1450. https://doi.org/10.3892/ijo.23.5.1445
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