Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an HLA-A24 restricted manner

  • Authors:
    • Keiichi Kontani
    • Koji Teramoto
    • Yoshitomo Ozaki
    • Takuya Fujita
    • Noriaki Tezuka
    • Satoru Sawai
    • Hiroyoshi Watanabe
    • Shozo Fujino
    • Hiroyasu Yokomise
    • Iwao Ohkubo
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijo.25.6.1537
  • Pages: 1537-1542
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Abstract

We previously reported that 90K/Mac-2 binding protein (M2BP) is highly expressed in lung cancer and that M2BP-specific immunity was observed in many patients with lung cancer. These findings suggested the possibility of using M2BP as a target antigen in cancer immunotherapy. In this study, we selected 11 peptides derived from M2BP with an HLA-A24 binding motif and analyzed their ability to induce M2BP-specific cytotoxic T lymphocytes (CTL). CTLs were generated with the M2BP-derived peptides from peripheral blood CD8-positive T lymphocytes of HLA-A24-positive healthy donors in multiple in vitro stimulations. Two CTLs, one induced with M2BP241-250 (GYCASLFAIL) and the other with M2BP568-576 (GFRTVIRPF), produced interferon-γ in response to HLA-A24-positive TISI cells pulsed with the same peptide used for the in vitro stimulation. Although the CTLs induced with M2BP241-250 reacted with both peptide-pulsed TISI cells and BT20 cells expressing both M2BP and HLA-A24, the CTLs induced with M2BP568-576 did not react with BT20 cells. The cytokine production was blocked by antibodies against HLA class I in CTLs induced using M2BP241-250, but not in CTLs induced using M2BP568-576. These findings suggest that M2BP241-250 is naturally processed from the native M2BP molecule in cancer cells and recognized by M2BP-specific CTLs in an HLA-A24 restriction. An M2BP-derived CTL epitope with an HLA-A24 binding motif was identified for the first time in this study, and it is expected to be useful as a target antigenic epitope in clinical immunotherapy for lung cancer.

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December 2004
Volume 25 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kontani K, Teramoto K, Ozaki Y, Fujita T, Tezuka N, Sawai S, Watanabe H, Fujino S, Yokomise H, Ohkubo I, Ohkubo I, et al: Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an HLA-A24 restricted manner. Int J Oncol 25: 1537-1542, 2004
APA
Kontani, K., Teramoto, K., Ozaki, Y., Fujita, T., Tezuka, N., Sawai, S. ... Ohkubo, I. (2004). Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an HLA-A24 restricted manner. International Journal of Oncology, 25, 1537-1542. https://doi.org/10.3892/ijo.25.6.1537
MLA
Kontani, K., Teramoto, K., Ozaki, Y., Fujita, T., Tezuka, N., Sawai, S., Watanabe, H., Fujino, S., Yokomise, H., Ohkubo, I."Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an HLA-A24 restricted manner". International Journal of Oncology 25.6 (2004): 1537-1542.
Chicago
Kontani, K., Teramoto, K., Ozaki, Y., Fujita, T., Tezuka, N., Sawai, S., Watanabe, H., Fujino, S., Yokomise, H., Ohkubo, I."Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes in an HLA-A24 restricted manner". International Journal of Oncology 25, no. 6 (2004): 1537-1542. https://doi.org/10.3892/ijo.25.6.1537