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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2004 Volume 25 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 2004 Volume 25 Issue 6

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Article

HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies

  • Authors:
    • Coya Tapia
    • Peter Schraml
    • Ronald Simon
    • Khawla S. Al-Kuraya
    • Robert Maurer
    • Martina Mirlacher
    • Hedvika Novotny
    • Hanspeter Spichtin
    • Michael J. Mihatsch
    • Guido Sauter

  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, University of Basel, Basel, Switzerland
  • Pages: 1551-1557
    |
    Published online on: December 1, 2004
       https://doi.org/10.3892/ijo.25.6.1551
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Abstract

Due to the central role in predicting response to herceptin and possibly also other anticancer drugs, accurate and reproducible detection of the HER2 status is important. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are the most commonly used methods for HER2 analysis. It is a disadvantage of FISH that a fraction of cases remain not interpretable probably due to suboptimal tissue handling before analysis. To investigate a possible influence of tissue damage on the results of HER2 IHC we compared the HER2 IHC results obtained in tumors with and without interpretable FISH in a breast cancer tissue microarray. The HER2 IHC results differed greatly between 1551 tumors with interpretable HER2 FISH signals and 405 breast cancers showing no FISH signals. FISH informative tumors had an IHC score of 3+ in 12.6%, 2+ in 3% and 1+ in 9.2% of cases. FISH non-informative tumors showed significantly lower IHC scores (p<0.0001). They were IHC 3+ in 3.9%, 2+ in 3.7% and 1+ in 4.4% of cases. Overall, the data show that not only FISH but also IHC results are dependent on good tissue quality for successful analysis. Poor tissue quality can be easily identified in FISH analyses because of a lack of hybridization signals. Inappropriate tissue handling is more dangerous in IHC because an artificial lack of staining can be regarded as ‘negative’ result.

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Copy and paste a formatted citation
Spandidos Publications style
Tapia C, Schraml P, Simon R, Al-Kuraya KS, Maurer R, Mirlacher M, Novotny H, Spichtin H, Mihatsch MJ, Sauter G, Sauter G, et al: HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies. Int J Oncol 25: 1551-1557, 2004.
APA
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K.S., Maurer, R., Mirlacher, M. ... Sauter, G. (2004). HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies. International Journal of Oncology, 25, 1551-1557. https://doi.org/10.3892/ijo.25.6.1551
MLA
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K. S., Maurer, R., Mirlacher, M., Novotny, H., Spichtin, H., Mihatsch, M. J., Sauter, G."HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies". International Journal of Oncology 25.6 (2004): 1551-1557.
Chicago
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K. S., Maurer, R., Mirlacher, M., Novotny, H., Spichtin, H., Mihatsch, M. J., Sauter, G."HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies". International Journal of Oncology 25, no. 6 (2004): 1551-1557. https://doi.org/10.3892/ijo.25.6.1551
Copy and paste a formatted citation
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Spandidos Publications style
Tapia C, Schraml P, Simon R, Al-Kuraya KS, Maurer R, Mirlacher M, Novotny H, Spichtin H, Mihatsch MJ, Sauter G, Sauter G, et al: HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies. Int J Oncol 25: 1551-1557, 2004.
APA
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K.S., Maurer, R., Mirlacher, M. ... Sauter, G. (2004). HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies. International Journal of Oncology, 25, 1551-1557. https://doi.org/10.3892/ijo.25.6.1551
MLA
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K. S., Maurer, R., Mirlacher, M., Novotny, H., Spichtin, H., Mihatsch, M. J., Sauter, G."HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies". International Journal of Oncology 25.6 (2004): 1551-1557.
Chicago
Tapia, C., Schraml, P., Simon, R., Al-Kuraya, K. S., Maurer, R., Mirlacher, M., Novotny, H., Spichtin, H., Mihatsch, M. J., Sauter, G."HER2 analysis in breast cancer: Reduced immunoreactivity in FISH non-informative cancer biopsies". International Journal of Oncology 25, no. 6 (2004): 1551-1557. https://doi.org/10.3892/ijo.25.6.1551
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