Induction of apoptosis in non-small lung carcinoma cell line (H1299) by combination of anti-asthma drugs with gemcitabine and cisplatin

  • Authors:
    • O. Merimsky
    • L. Hirsh
    • A. Dantes
    • A. Land-Bracha
    • B. S. Suh
    • A. Amsterdam
  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/ijo.26.2.475
  • Pages: 475-482
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gemcitabine and cisplatin are commonly used in chemotherapy, however, these drugs may cause severe cytotoxic side effects. Theophylline and aminophylline are commonly used as anti-asthma drugs and can block anti-phosphodiesterase activity. We examined whether these methylxanthins could effect lung cancer cell survival and synergise with gemcitabine and cisplatin to induce apoptosis. We found that theophylline induced apoptosis in the cultured H1299 cell line already at concentrations of 30 µg/ml, reaching an ED50% at 100 µg/ml. In contrast, aminophylline induced apoptosis at concentrations of 300 µg/ml and 17% apoptosis was evident at concentrations as high as 900 µg/ml, which is a lethal dose for in vivo treatment. Cisplatin induced apoptosis with ED50% of 0.8 µg/ml, while gemcitabine induced apoptosis with ED50% of 20 ng/ml. Using a combination of 20 µg/ml of theophylline (calculated as an effective but not toxic anti-asthma drug) with 10 ng/ml gemcitabine or with 0.3 µg/ml cisplatin significantly elevated incidence of apoptosis compared to gemcitabine or cisplatin alone at similar concentrations. In contrast, an observed synergistic effect between aminophylline and gemcitabine was evident only at concentrations of 80 µg/ml and 10 ng/ml respectively. However, no effect was apparent in combination doses of aminophylline (80 µg/ml) with cisplatin (0.3 µg/ml). The combined treatments involved reduction in the intracellular level of the anti-apoptotic Bcl-2 gene product. This corresponded with the extent of apoptosis induced by the various drug combinations. Thus, theophylline is significantly more effective than aminophylline in increasing the sensitivity of the H1299 lung cancer cells to the induction of cell death by gemcitabine and cisplatin. Thus, combination of theophylline with these drugs may permit a reduction in the effective dose needed in chemotherapy treatment of lung cancer patients.

Related Articles

Journal Cover

February 2005
Volume 26 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Merimsky O, Hirsh L, Dantes A, Land-Bracha A, Suh BS and Amsterdam A: Induction of apoptosis in non-small lung carcinoma cell line (H1299) by combination of anti-asthma drugs with gemcitabine and cisplatin. Int J Oncol 26: 475-482, 2005
APA
Merimsky, O., Hirsh, L., Dantes, A., Land-Bracha, A., Suh, B.S., & Amsterdam, A. (2005). Induction of apoptosis in non-small lung carcinoma cell line (H1299) by combination of anti-asthma drugs with gemcitabine and cisplatin. International Journal of Oncology, 26, 475-482. https://doi.org/10.3892/ijo.26.2.475
MLA
Merimsky, O., Hirsh, L., Dantes, A., Land-Bracha, A., Suh, B. S., Amsterdam, A."Induction of apoptosis in non-small lung carcinoma cell line (H1299) by combination of anti-asthma drugs with gemcitabine and cisplatin". International Journal of Oncology 26.2 (2005): 475-482.
Chicago
Merimsky, O., Hirsh, L., Dantes, A., Land-Bracha, A., Suh, B. S., Amsterdam, A."Induction of apoptosis in non-small lung carcinoma cell line (H1299) by combination of anti-asthma drugs with gemcitabine and cisplatin". International Journal of Oncology 26, no. 2 (2005): 475-482. https://doi.org/10.3892/ijo.26.2.475