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In vivo analyses of UV-irradiation-induced p53 promoter binding using a novel quantitative real-time PCR assay

  • Authors:
    • Jenny C. Potratz
    • Barbara Mlody
    • Wolfgang E. Berdel
    • Hubert Serve
    • Carsten Müller-Tidow

  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/ijo.26.2.493
  • Pages: 493-498
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Abstract

The p53 tumor suppressor protein mediates cell cycle arrest and apoptosis through transactivation of downstream target genes. While many target genes have been identified to date, the mechanisms and time course of their induction are still unclear. We investigated the kinetics of p53 binding to the p21CIP1, MDM2, BAX and PIG3 promoters in vivo using a novel quantitative real-time chromatin immunoprecipitation-PCR assay. Our results demonstrate distinct kinetics of p53 promoter binding dependent on the target gene promoters. The timed induction of target genes due to genotoxic stress is likely to play a pivotal role for the divergent functions of p53.

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Journal Cover

February 2005
Volume 26 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
Potratz JC, Mlody B, Berdel WE, Serve H and Müller-Tidow C: In vivo analyses of UV-irradiation-induced p53 promoter binding using a novel quantitative real-time PCR assay. Int J Oncol 26: 493-498, 2005
APA
Potratz, J.C., Mlody, B., Berdel, W.E., Serve, H., & Müller-Tidow, C. (2005). In vivo analyses of UV-irradiation-induced p53 promoter binding using a novel quantitative real-time PCR assay. International Journal of Oncology, 26, 493-498. https://doi.org/10.3892/ijo.26.2.493
MLA
Potratz, J. C., Mlody, B., Berdel, W. E., Serve, H., Müller-Tidow, C."In vivo analyses of UV-irradiation-induced p53 promoter binding using a novel quantitative real-time PCR assay". International Journal of Oncology 26.2 (2005): 493-498.
Chicago
Potratz, J. C., Mlody, B., Berdel, W. E., Serve, H., Müller-Tidow, C."In vivo analyses of UV-irradiation-induced p53 promoter binding using a novel quantitative real-time PCR assay". International Journal of Oncology 26, no. 2 (2005): 493-498. https://doi.org/10.3892/ijo.26.2.493