Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia

  • Authors:
    • Ravi Kothapalli
    • Susan B. Nyland
    • Irina Kusmartseva
    • Ratna D. Bailey
    • Tylee M. McKeown
    • Thomas P. Loughran
  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/ijo.26.2.529
  • Pages: 529-535
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Abstract

Large granular lymphocyte (LGL) leukemia is a lymphoproliferative disease often associated with autoimmune disorders such as rheumatoid arthritis. High levels of soluble Fas ligand have been implicated in development of chronic neutropenia. However, a comprehensive analysis of constitutive chemokine and lymphokine production in LGL leukemia has not previously been reported. Here, we utilized RNase protection assays and enzyme-linked immunosorbent assays (ELISAs) to address this question. RANTES, IL-8, MIP-1α, MIP-1β, IL-1β, IL-10, IL-12 p35, IL-18, IFN-γ and IL-1Ra were the cytokine transcripts expressed in elevated levels from RNA of peripheral blood mononuclear cells of LGL leukemia patients. Confirmatory ELISAs indicated that sera from LGL leukemia patients have elevated levels of RANTES, MIP-1β, IL-18, and to a lesser extent IL-8 and IL-1Ra. This pattern of cytokine upregulation is similar to that seen in some chronic infections or in autoimmune diseases, thus characterizing LGL leukemia as a proinflammatory disorder.

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February 2005
Volume 26 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kothapalli R, Nyland SB, Kusmartseva I, Bailey RD, McKeown TM and Loughran TP: Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia. Int J Oncol 26: 529-535, 2005
APA
Kothapalli, R., Nyland, S.B., Kusmartseva, I., Bailey, R.D., McKeown, T.M., & Loughran, T.P. (2005). Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia. International Journal of Oncology, 26, 529-535. https://doi.org/10.3892/ijo.26.2.529
MLA
Kothapalli, R., Nyland, S. B., Kusmartseva, I., Bailey, R. D., McKeown, T. M., Loughran, T. P."Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia". International Journal of Oncology 26.2 (2005): 529-535.
Chicago
Kothapalli, R., Nyland, S. B., Kusmartseva, I., Bailey, R. D., McKeown, T. M., Loughran, T. P."Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia". International Journal of Oncology 26, no. 2 (2005): 529-535. https://doi.org/10.3892/ijo.26.2.529