Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach

  • Authors:
    • Hirofumi Koike
    • Katsuhiro Uzawa
    • Dai Nakashima
    • Ken Shimada
    • Yoshikuni Kato
    • Morihiro Higo
    • Yukinao Kouzu
    • Yosuke Endo
    • Atsushi Kasamatsu
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijo.27.1.59
  • Pages: 59-67
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A number of protein markers for oral cancer are still not applicable in large populations. Proteomic technologies provide excellent tools for rapid screening of a large number of potential biomarkers in malignant cells. To gain insight into the molecular mechanisms of carcinogenesis and to identify potential biomarkers for oral squamous cell carcinomas (OSCCs), we performed proteomic profiling between human normal oral keratinocytes (HNOKs) and OSCC-derived cell lines (HSC-2 and HSC-3) using fluorescent two-dimensional difference in-gel electrophoresis. Proteins with a ≥2-fold change in expression were considered significant. The spots of interest were digested and identified by matrix-assisted laser desorption/ionization time-of-flight peptide mass finger-printing. Twenty-two proteins were identified as differentially expressed between the HNOKs and OSCC-derived cell lines. Of these, 9 spots were up-regulated and 13 were down-regulated in OSCC-derived cell lines compared to the HNOKs. These spots included the cancer-related proteins; annexin A1, heat shock protein 27, lamin A/C, interleukin 1 receptor antagonist, serine proteinase inhibitor clade B5, stathmin 1, and superoxide dismutase 2. Our results are a first step toward identifying a protein profile of HNOKs and OSCC-derived cell lines. The identified proteins in this experiment may be used in future studies of carcinogenesis or as diagnostic markers and therapeutic targets for OSCC.

Related Articles

Journal Cover

July 2005
Volume 27 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Koike H, Uzawa K, Nakashima D, Shimada K, Kato Y, Higo M, Kouzu Y, Endo Y, Kasamatsu A, Tanzawa H, Tanzawa H, et al: Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach. Int J Oncol 27: 59-67, 2005
APA
Koike, H., Uzawa, K., Nakashima, D., Shimada, K., Kato, Y., Higo, M. ... Tanzawa, H. (2005). Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach. International Journal of Oncology, 27, 59-67. https://doi.org/10.3892/ijo.27.1.59
MLA
Koike, H., Uzawa, K., Nakashima, D., Shimada, K., Kato, Y., Higo, M., Kouzu, Y., Endo, Y., Kasamatsu, A., Tanzawa, H."Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach". International Journal of Oncology 27.1 (2005): 59-67.
Chicago
Koike, H., Uzawa, K., Nakashima, D., Shimada, K., Kato, Y., Higo, M., Kouzu, Y., Endo, Y., Kasamatsu, A., Tanzawa, H."Identification of differentially expressed proteins in oral squamous cell carcinoma using a global proteomic approach". International Journal of Oncology 27, no. 1 (2005): 59-67. https://doi.org/10.3892/ijo.27.1.59