Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer

Hara,Miho; Matsueda,Satoko; Tamura,Mayumi; Takedatsu,Hiroko; Tanaka,Masahiro; Kawano,Kouichiro; Mochizuki,Kazuo; Kamura,Toshiharu; Itoh,Kyogo; Harada,Mamoru

doi:10.3892/ijo.27.5.1371

November 2005 Volume 27 Issue 5

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November 2005 Volume 27 Issue 5

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Article

Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer

Authors:
- Miho Hara
- Satoko Matsueda
- Mayumi Tamura
- Hiroko Takedatsu
- Masahiro Tanaka
- Kouichiro Kawano
- Kazuo Mochizuki
- Toshiharu Kamura
- Kyogo Itoh
- Mamoru Harada
View Affiliations / Copyright

Affiliations: Department of Immunology, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan
Pages: 1371-1379
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Published online on: November 1, 2005

https://doi.org/10.3892/ijo.27.5.1371
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Abstract

Human papillomavirus 16 (HPV16)-E6 and -E7 proteins are considered to be appropriate targets in specific immunotherapy for cervical cancer. In this study, we attempted to identify epitope peptides from the HPV16-E6 protein that have the potential to generate cytotoxic T-lymphocytes (CTLs) toward human leukocyte antigen (HLA)-A24+ cervical cancer. Two HPV16-E6 peptides at positions 75-83 and 91-100 effectively induced peptide-specific CTLs from peripheral blood mononuclear cells of HLA-A24+ cervical cancer patients. These HPV16-E6 peptide-induced CTLs showed cytotoxicity against HLA-A24+ and HPV16-E6 protein-expressing cervical cancer cells. Experiments with blocking antibodies and cold inhibition targets revealed that the cytotoxicity was mainly dependent on peptide-specific and CD8+ T cells. In addition, based on our observation that induction of immunoglobulin G (IgG) reactive to administered CTL-directed peptides is correlated with clinical responses, we attempted to detect IgG reactive to HPV16-E6 peptides in the plasma of cancer patients. As a result, IgGs reactive to the HPV16-E691-100 peptide were detected in 4 of 12 cervical cancer patients. These results indicate that these HPV16-E6-derived peptides are good candidates in peptide-based immunotherapy for HLA-A24+ cervical cancer patients.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer

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