Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer

  • Authors:
    • Miho Hara
    • Satoko Matsueda
    • Mayumi Tamura
    • Hiroko Takedatsu
    • Masahiro Tanaka
    • Kouichiro Kawano
    • Kazuo Mochizuki
    • Toshiharu Kamura
    • Kyogo Itoh
    • Mamoru Harada
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijo.27.5.1371
  • Pages: 1371-1379
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Abstract

Human papillomavirus 16 (HPV16)-E6 and -E7 proteins are considered to be appropriate targets in specific immunotherapy for cervical cancer. In this study, we attempted to identify epitope peptides from the HPV16-E6 protein that have the potential to generate cytotoxic T-lymphocytes (CTLs) toward human leukocyte antigen (HLA)-A24+ cervical cancer. Two HPV16-E6 peptides at positions 75-83 and 91-100 effectively induced peptide-specific CTLs from peripheral blood mononuclear cells of HLA-A24+ cervical cancer patients. These HPV16-E6 peptide-induced CTLs showed cytotoxicity against HLA-A24+ and HPV16-E6 protein-expressing cervical cancer cells. Experiments with blocking antibodies and cold inhibition targets revealed that the cytotoxicity was mainly dependent on peptide-specific and CD8+ T cells. In addition, based on our observation that induction of immunoglobulin G (IgG) reactive to administered CTL-directed peptides is correlated with clinical responses, we attempted to detect IgG reactive to HPV16-E6 peptides in the plasma of cancer patients. As a result, IgGs reactive to the HPV16-E691-100 peptide were detected in 4 of 12 cervical cancer patients. These results indicate that these HPV16-E6-derived peptides are good candidates in peptide-based immunotherapy for HLA-A24+ cervical cancer patients.

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November 2005
Volume 27 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hara M, Matsueda S, Tamura M, Takedatsu H, Tanaka M, Kawano K, Mochizuki K, Kamura T, Itoh K, Harada M, Harada M, et al: Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer. Int J Oncol 27: 1371-1379, 2005
APA
Hara, M., Matsueda, S., Tamura, M., Takedatsu, H., Tanaka, M., Kawano, K. ... Harada, M. (2005). Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer. International Journal of Oncology, 27, 1371-1379. https://doi.org/10.3892/ijo.27.5.1371
MLA
Hara, M., Matsueda, S., Tamura, M., Takedatsu, H., Tanaka, M., Kawano, K., Mochizuki, K., Kamura, T., Itoh, K., Harada, M."Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer". International Journal of Oncology 27.5 (2005): 1371-1379.
Chicago
Hara, M., Matsueda, S., Tamura, M., Takedatsu, H., Tanaka, M., Kawano, K., Mochizuki, K., Kamura, T., Itoh, K., Harada, M."Identification of human papillomavirus 16-E6 protein-derived peptides with the potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ cervical cancer". International Journal of Oncology 27, no. 5 (2005): 1371-1379. https://doi.org/10.3892/ijo.27.5.1371