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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2005 Volume 27 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 2005 Volume 27 Issue 6

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Article

A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation

  • Authors:
    • Ramanathan Arvind
    • Hiroaki Shimamoto
    • Fumio Momose
    • Teruo Amagasa
    • Ken Omura
    • Nobuo Tsuchida

  • View Affiliations / Copyright

    Affiliations: Department of Molecular Cellular Oncology and Microbiology, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 113-8549 Tokyo, Japan
  • Pages: 1499-1504
    |
    Published online on: December 1, 2005
       https://doi.org/10.3892/ijo.27.6.1499
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Abstract

The EGFR/Ras/Raf/MEK/ERK pathway is a major pathway involved in the control of growth signals, cell survival and differentiation. Mutations of signaling components, such as EGFR (c-erbB1), Ras, and B-Raf, have been shown to play roles in the genesis of human cancer, while point mutation of ERK has not been reported. In this study, we present evidence for a mutation in an oral squamous cell carcinoma cell line, HSC6. PCR-amplification of cDNA, cloning and sequencing resulted in the identification of glutamic acid to lysine substitution at codon 322 (E322K) that occurred in the common docking (CD) domain of ERK2. The mutant protein contributed towards faster-migration in SDS-PAGE, and constitutive phosphorylation in a MEK-dependent manner. The transient transfection of the mutant ERK2 in 293T cells resulted in the expression of the same faster-migrating band in SDS-PAGE as was detected in HSC6 cells, which was preferentially phosphorylated relative to endogenous wild-type ERK2. The present study is the first to report ERK2 substitution mutation in a human cancer cell line which resulted in constitutive phosphorylation.

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Copy and paste a formatted citation
Spandidos Publications style
Arvind R, Shimamoto H, Momose F, Amagasa T, Omura K and Tsuchida N: A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation. Int J Oncol 27: 1499-1504, 2005.
APA
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., & Tsuchida, N. (2005). A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation. International Journal of Oncology, 27, 1499-1504. https://doi.org/10.3892/ijo.27.6.1499
MLA
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., Tsuchida, N."A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation". International Journal of Oncology 27.6 (2005): 1499-1504.
Chicago
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., Tsuchida, N."A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation". International Journal of Oncology 27, no. 6 (2005): 1499-1504. https://doi.org/10.3892/ijo.27.6.1499
Copy and paste a formatted citation
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Spandidos Publications style
Arvind R, Shimamoto H, Momose F, Amagasa T, Omura K and Tsuchida N: A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation. Int J Oncol 27: 1499-1504, 2005.
APA
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., & Tsuchida, N. (2005). A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation. International Journal of Oncology, 27, 1499-1504. https://doi.org/10.3892/ijo.27.6.1499
MLA
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., Tsuchida, N."A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation". International Journal of Oncology 27.6 (2005): 1499-1504.
Chicago
Arvind, R., Shimamoto, H., Momose, F., Amagasa, T., Omura, K., Tsuchida, N."A mutation in the common docking domain of ERK2 in a human cancer cell line, which was associated with its constitutive phosphorylation". International Journal of Oncology 27, no. 6 (2005): 1499-1504. https://doi.org/10.3892/ijo.27.6.1499
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