Gene expression profiles of oral leukoplakia and carcinoma: Genome-wide comparison analysis using oligonucleotide microarray technology

  • Authors:
    • Takeshi Odani
    • Daisuke Ito
    • Ming-Heng Li
    • Ai Kawamata
    • Tomohide Isobe
    • Masayasu Iwase
    • Masao Nagumo
  • View Affiliations

  • Published online on: March 1, 2006     https://doi.org/10.3892/ijo.28.3.619
  • Pages: 619-624
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Although leukoplakia is the most common precancerous lesion of the oral cavity, its molecular biological properties are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis and malignant transformation using oligonucleotide microarray technology. The expression profiles of 8,800 genes in human oral leukoplakia (n=4) and oral squamous cell carcinoma (OSCC) (n=2) were analyzed using the Affymetrix GeneChip® system and the results were confirmed with RT-PCR. Eight genes were up-regulated (>2.0-fold) and ten were down-regulated (<0.5-fold) in all leukoplakias analyzed with the GeneChip. In particular, loricrin and keratins displayed greater differences between normal tissue and leukoplakia. Some of the 18 alternatively expressed genes were markedly down-regulated in squamous cell carcinoma compared with leukoplakia. Our data suggested that gene abnormalities in cytoskeleton network components might be responsible for the development and progression of oral leukoplakia.

Related Articles

Journal Cover

March 2006
Volume 28 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Odani T, Ito D, Li M, Kawamata A, Isobe T, Iwase M and Nagumo M: Gene expression profiles of oral leukoplakia and carcinoma: Genome-wide comparison analysis using oligonucleotide microarray technology. Int J Oncol 28: 619-624, 2006
APA
Odani, T., Ito, D., Li, M., Kawamata, A., Isobe, T., Iwase, M., & Nagumo, M. (2006). Gene expression profiles of oral leukoplakia and carcinoma: Genome-wide comparison analysis using oligonucleotide microarray technology. International Journal of Oncology, 28, 619-624. https://doi.org/10.3892/ijo.28.3.619
MLA
Odani, T., Ito, D., Li, M., Kawamata, A., Isobe, T., Iwase, M., Nagumo, M."Gene expression profiles of oral leukoplakia and carcinoma: Genome-wide comparison analysis using oligonucleotide microarray technology". International Journal of Oncology 28.3 (2006): 619-624.
Chicago
Odani, T., Ito, D., Li, M., Kawamata, A., Isobe, T., Iwase, M., Nagumo, M."Gene expression profiles of oral leukoplakia and carcinoma: Genome-wide comparison analysis using oligonucleotide microarray technology". International Journal of Oncology 28, no. 3 (2006): 619-624. https://doi.org/10.3892/ijo.28.3.619