Ganoderma lucidum inhibits proliferation of human breast cancer cells by down-regulation of estrogen receptor and NF-κB signaling

  • Authors:
    • Jiahua Jiang
    • Veronika Slivova
    • Daniel Sliva
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijo.29.3.695
  • Pages:695-703
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Abstract

Ganoderma lucidum, an oriental medical mushroom, has been used in Asia for the prevention and treatment of a variety of diseases, including cancer. We have previously demonstrated that G. lucidum inhibits growth and induces cell cycle arrest at G0/G1 phase through the inhibition of Akt/NF-κB signaling in estrogen-independent human breast cancer cells. However, the molecular mechanism(s) responsible for the inhibitory effects of G. lucidum on the proliferation of estrogen-dependent (MCF-7) and estrogen-independent (MDA-MB-231) breast cancer cells remain to be elucidated. Here, we show that G. lucidum inhibited the proliferation of breast cancer MCF-7 and MDA-MB-231 cells by the modulation of the estrogen receptor (ER) and NF-κB signaling. Thus, G. lucidum down-regulated the expression of ERα in MCF-7 cells but did not effect the expression of ERβ in MCF-7 and MDA-MB-231 cells. In addition, G. lucidum inhibited estrogen-dependent as well as constitutive transactivation activity of ER through estrogen response element (ERE) in a reporter gene assay. G. lucidum decreased TNF-α-induced (MCF-7) as well as constitutive (MDA-MB-231) activity of NF-κB. The inhibition of ER and NF-κB pathways resulted in the down-regulation of expression of c-myc, finally suppressing proliferation of estrogen-dependent as well as estrogen-independent cancer cells. Collectively, these results suggest that G. lucidum inhibits proliferation of human breast cancer cells and contain biologically active compounds with specificity against estrogen receptor and NF-κB signaling, and implicate G. lucidum as a suitable herb for chemoprevention and chemotherapy of breast cancer.

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September 2006
Volume 29 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
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APA
Jiang, J., Slivova, V., & Sliva, D. (2006). Ganoderma lucidum inhibits proliferation of human breast cancer cells by down-regulation of estrogen receptor and NF-κB signaling. International Journal of Oncology, 29, 695-703. https://doi.org/10.3892/ijo.29.3.695
MLA
Jiang, J., Slivova, V., Sliva, D."Ganoderma lucidum inhibits proliferation of human breast cancer cells by down-regulation of estrogen receptor and NF-κB signaling". International Journal of Oncology 29.3 (2006): 695-703.
Chicago
Jiang, J., Slivova, V., Sliva, D."Ganoderma lucidum inhibits proliferation of human breast cancer cells by down-regulation of estrogen receptor and NF-κB signaling". International Journal of Oncology 29, no. 3 (2006): 695-703. https://doi.org/10.3892/ijo.29.3.695