Elevated cell migration, invasion and tumorigenicity in human KB carcinoma cells transfected with COX-2 cDNA

  • Authors:
    • Kazuki Takaoka
    • Hiromitsu Kishimoto
    • Emi Segawa
    • Susumu Hashitani
    • Yusuke Zushi
    • Kazuma Noguchi
    • Kazunari Sakurai
    • Masahiro Urade
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1095
  • Pages: 1095-1101
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Abstract

In order to investigate the involvement of cyclooxygenase (COX)-2 in cell growth and invasion of oral cancer, a human epidermoid carcinoma cell line KB minimally expressing COX-2 protein was transfected with COX-2 cDNA and these activities were compared with mock-transfected KB in vitro and in vivo. KB/COX-2 clones showed a similar growth rate in vitro compared to KB/neo clones, but demonstrated significantly increased PGE2 production, cell migration and invasion. These KB/COX-2 clones markedly expressed MMP-9, pro-MMP-2 and activated-MMP-2 as compared to KB/neo clones in gelatin zymography. Western blot analysis showed that expression of MT1-MMP, Rho and Rac 1 in KB/COX-2 clones were stronger than that in KB/neo clones, but expression of TIMP-1 and TIMP-2 were weaker in KB/COX-2 clones than in KB/neo clones. When these cells were inoculated subcutaneously into nude mice, tumorigenicity and tumor growth were significantly elevated in KB/COX-2 tumors than in KB/neo tumors, and the gelatinase activity was much stronger in KB/COX-2 tumor tissues than in KB/neo tumor tissues in film in situ zymography. The orthotopic inoculation of cells to the oral floor showed that local invasion was pronounced in KB/COX-2 tumors. These results indicated that overexpression of COX-2 elevated tumorigenicity, tumor growth and invasion of human KB carcinoma cells via up-regulated MMP and Rho family small GTPases and down-regulated TIMP activities.

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November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Takaoka K, Kishimoto H, Segawa E, Hashitani S, Zushi Y, Noguchi K, Sakurai K and Urade M: Elevated cell migration, invasion and tumorigenicity in human KB carcinoma cells transfected with COX-2 cDNA. Int J Oncol 29: 1095-1101, 2006
APA
Takaoka, K., Kishimoto, H., Segawa, E., Hashitani, S., Zushi, Y., Noguchi, K. ... Urade, M. (2006). Elevated cell migration, invasion and tumorigenicity in human KB carcinoma cells transfected with COX-2 cDNA. International Journal of Oncology, 29, 1095-1101. https://doi.org/10.3892/ijo.29.5.1095
MLA
Takaoka, K., Kishimoto, H., Segawa, E., Hashitani, S., Zushi, Y., Noguchi, K., Sakurai, K., Urade, M."Elevated cell migration, invasion and tumorigenicity in human KB carcinoma cells transfected with COX-2 cDNA". International Journal of Oncology 29.5 (2006): 1095-1101.
Chicago
Takaoka, K., Kishimoto, H., Segawa, E., Hashitani, S., Zushi, Y., Noguchi, K., Sakurai, K., Urade, M."Elevated cell migration, invasion and tumorigenicity in human KB carcinoma cells transfected with COX-2 cDNA". International Journal of Oncology 29, no. 5 (2006): 1095-1101. https://doi.org/10.3892/ijo.29.5.1095