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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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July 1993 Volume 3 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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July 1993 Volume 3 Issue 1

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Article

IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS

  • Authors:
    • KK WONG
    • CH MOK
    • JT WELSH
    • M MCCLELLAND
    • SW TSAO
    • RS BERKOWITZ

  • View Affiliations / Copyright

    Affiliations: BRIGHAM & WOMENS HOSP,DEPT OBSTET GYNECOL & REPROD BIOL,GYNECOL ONCOL LAB,BOSTON,MA 02115. CALIF INST BIOL RES,LA JOLLA,CA 92037.
  • Pages: 13-17
    |
    Published online on: July 1, 1993
       https://doi.org/10.3892/ijo.3.1.13
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Abstract

Using arbitrarily primed PCR fingerprinting of RNA (RAP), we have analyzed RNAs prepared from two normal ovarian surface epithelial cell cultures, two normal mesothelial cell cultures, and eight independent ovarian carcinoma cell lines. Each arbitrarily chosen primer gave a unique fingerprint of about 30 cDNA products. Most of the cDNA products produced by any particular primer were shared between all cell lines. However, one primer detected a cDNA PCR product that was absent in all eight ovarian carcinoma cell lines but present in all normal cell cultures. We have cloned and sequenced the DNA fragment that distinguishes normal from ovarian carcinoma cell lines. The DNA sequence has one continuous open reading frame throughout which indicates that it may be from a translated gene. Furthermore, we confirmed the differential expression of the gene by Northern blot analysis. We also observed that the intensity of the band in the RNA fingerprint correlates with the expression level of the corresponding RNA. These results further demonstrate the ability of RAP to detect differentially expressed genes in a quantitative manner and demonstrated the application of RAP for the detection of differential gene expression during carcinogenesis.

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Copy and paste a formatted citation
Spandidos Publications style
WONG K, MOK C, WELSH J, MCCLELLAND M, TSAO S and BERKOWITZ R: IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS. Int J Oncol 3: 13-17, 1993.
APA
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., & BERKOWITZ, R. (1993). IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS. International Journal of Oncology, 3, 13-17. https://doi.org/10.3892/ijo.3.1.13
MLA
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., BERKOWITZ, R."IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS". International Journal of Oncology 3.1 (1993): 13-17.
Chicago
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., BERKOWITZ, R."IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS". International Journal of Oncology 3, no. 1 (1993): 13-17. https://doi.org/10.3892/ijo.3.1.13
Copy and paste a formatted citation
x
Spandidos Publications style
WONG K, MOK C, WELSH J, MCCLELLAND M, TSAO S and BERKOWITZ R: IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS. Int J Oncol 3: 13-17, 1993.
APA
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., & BERKOWITZ, R. (1993). IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS. International Journal of Oncology, 3, 13-17. https://doi.org/10.3892/ijo.3.1.13
MLA
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., BERKOWITZ, R."IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS". International Journal of Oncology 3.1 (1993): 13-17.
Chicago
WONG, K., MOK, C., WELSH, J., MCCLELLAND, M., TSAO, S., BERKOWITZ, R."IDENTIFICATION OF DIFFERENTIALLY EXPRESSED RNA IN HUMAN OVARIAN-CARCINOMA CELLS BY ARBITRARILY PRIMED PCR FINGERPRINTING OF TOTAL RNAS". International Journal of Oncology 3, no. 1 (1993): 13-17. https://doi.org/10.3892/ijo.3.1.13
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