REVERSAL OF DRUG-RESISTANCE BY EXPOSURE OF MCF-7 OR MCF-7 CDDP HUMAN BREAST-CARCINOMA CELLS TO SR-4233 OR ETANIDAZOLE UNDER HYPOXIC CONDITIONS

  • Authors:
    • T KUSUMOTO
    • BA TEICHER
    • SA HOLDEN
    • CN COLEMAN
    • SD LIU
    • EA SOTOMAYOR
  • View Affiliations

  • Published online on: August 1, 1993     https://doi.org/10.3892/ijo.3.2.205
  • Pages: 205-211
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Abstract

Drug resistance is a major problem in cancer therapy. The MCF-7/CDDP cell line, a subline of the MCF-7 human breast carcinoma cell line which is resistant to cis-diamminedichloroplatinum(II), is also resistant to carboplatin, D-tetraplatin and to a lesser degree to melphalan, thiotepa and BCNU compared with the MCF-7 parental cell line. This resistance persists both under normally oxygenated conditions and after 2 h of exposure to hypoxic conditions prior to exposure to the antitumor alkylating agents under normally oxygenated conditions. When the MCF-7 parental cells and MCF-7/CDDP cells were treated with SR-4233 (20 muM) or etanidazole (5 mM) for 2 h prior to and during exposure to the antitumor alkylating agents there was no change in the sensitivity and resistance patterns of the cell lines. However, if the MCF-7 parental cells and the MCF-7/CDDP cells were exposed to SR-4233 (20 muM) or etanidazole (5 mM) for 2 h under hypoxic conditions followed by release of the hypoxia and exposure to the antitumor alkylating agents for 1 h under normally oxygenated conditions the resistance of the MCF-7/CDDP was reversed so that both the MCF-7 parental and MCF-7/CDDP cell lines were essentially equally sensitive to the six antitumor alkylating agents. These results indicate that non-cytotoxic concentrations of modulators such as SR-4233 or etanidazole may be useful in reversing resistance to the

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August 1993
Volume 3 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
KUSUMOTO T, TEICHER B, HOLDEN S, COLEMAN C, LIU S and SOTOMAYOR E: REVERSAL OF DRUG-RESISTANCE BY EXPOSURE OF MCF-7 OR MCF-7 CDDP HUMAN BREAST-CARCINOMA CELLS TO SR-4233 OR ETANIDAZOLE UNDER HYPOXIC CONDITIONS. Int J Oncol 3: 205-211, 1993.
APA
KUSUMOTO, T., TEICHER, B., HOLDEN, S., COLEMAN, C., LIU, S., & SOTOMAYOR, E. (1993). REVERSAL OF DRUG-RESISTANCE BY EXPOSURE OF MCF-7 OR MCF-7 CDDP HUMAN BREAST-CARCINOMA CELLS TO SR-4233 OR ETANIDAZOLE UNDER HYPOXIC CONDITIONS. International Journal of Oncology, 3, 205-211. https://doi.org/10.3892/ijo.3.2.205
MLA
KUSUMOTO, T., TEICHER, B., HOLDEN, S., COLEMAN, C., LIU, S., SOTOMAYOR, E."REVERSAL OF DRUG-RESISTANCE BY EXPOSURE OF MCF-7 OR MCF-7 CDDP HUMAN BREAST-CARCINOMA CELLS TO SR-4233 OR ETANIDAZOLE UNDER HYPOXIC CONDITIONS". International Journal of Oncology 3.2 (1993): 205-211.
Chicago
KUSUMOTO, T., TEICHER, B., HOLDEN, S., COLEMAN, C., LIU, S., SOTOMAYOR, E."REVERSAL OF DRUG-RESISTANCE BY EXPOSURE OF MCF-7 OR MCF-7 CDDP HUMAN BREAST-CARCINOMA CELLS TO SR-4233 OR ETANIDAZOLE UNDER HYPOXIC CONDITIONS". International Journal of Oncology 3, no. 2 (1993): 205-211. https://doi.org/10.3892/ijo.3.2.205