IMPAIRED ACTIVATION OF TUMORICIDAL FUNCTION IN MACROPHAGES FROM MAMMARY-TUMOR BEARERS - THE ROLE OF IFN-GAMMA

  • Authors:
    • EM SOTOMAYOR
    • XF CHENG
    • YX FU
    • A HAWKINS
    • C CALDERON-HIGGINSON
    • DM LOPEZ
  • View Affiliations

  • Published online on: October 1, 1993     https://doi.org/10.3892/ijo.3.4.719
  • Pages: 719-727
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Abstract

The growth of the DI-DMBA-3 mammary adenocarcinoma in BALB/c mice results in a profound deficit in the tumoricidal function of their peritoneal elicited macrophages (PEM) after stimulation with lipopolysaccharide (LPS). The capacity of these macrophages to respond to stronger signals, such as a combination of LPS and low levels (5 U/ml) of interferon-gamma (IFN-gamma) is also impaired. Importantly, a combination of high levels (50 U/ml) of IFN-gamma with 10 mug/ml of LPS, is able to trigger a cytolytic response in macrophages from tumor bearing mice against mammary tumor target cells, indicating that their lytic machinery is intact. However, IFN-gamma production is severely diminished in T lymphocytes from tumor bearing mice as detected by ELISA, moreover, Northern blots revealed that the levels of IFN-gamma RNA are also decreased in T cells from tumor bearers. The cooperation between T cells and macrophages is mediated, at least in part, by IFN-gamma. Thus, mammary tumors have die potential to overcome host defenses, either by affecting the capacity of macrophages to respond adequately to activating agents, and/or by impairing the production of T-cell derived lymphokines important in macrophage activation for tumor killing.

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October 1993
Volume 3 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
SOTOMAYOR E, CHENG X, FU Y, HAWKINS A, CALDERON-HIGGINSON C and LOPEZ D: IMPAIRED ACTIVATION OF TUMORICIDAL FUNCTION IN MACROPHAGES FROM MAMMARY-TUMOR BEARERS - THE ROLE OF IFN-GAMMA. Int J Oncol 3: 719-727, 1993.
APA
SOTOMAYOR, E., CHENG, X., FU, Y., HAWKINS, A., CALDERON-HIGGINSON, C., & LOPEZ, D. (1993). IMPAIRED ACTIVATION OF TUMORICIDAL FUNCTION IN MACROPHAGES FROM MAMMARY-TUMOR BEARERS - THE ROLE OF IFN-GAMMA. International Journal of Oncology, 3, 719-727. https://doi.org/10.3892/ijo.3.4.719
MLA
SOTOMAYOR, E., CHENG, X., FU, Y., HAWKINS, A., CALDERON-HIGGINSON, C., LOPEZ, D."IMPAIRED ACTIVATION OF TUMORICIDAL FUNCTION IN MACROPHAGES FROM MAMMARY-TUMOR BEARERS - THE ROLE OF IFN-GAMMA". International Journal of Oncology 3.4 (1993): 719-727.
Chicago
SOTOMAYOR, E., CHENG, X., FU, Y., HAWKINS, A., CALDERON-HIGGINSON, C., LOPEZ, D."IMPAIRED ACTIVATION OF TUMORICIDAL FUNCTION IN MACROPHAGES FROM MAMMARY-TUMOR BEARERS - THE ROLE OF IFN-GAMMA". International Journal of Oncology 3, no. 4 (1993): 719-727. https://doi.org/10.3892/ijo.3.4.719