EXPRESSION OF HEPATOCYTE GROWTH FACTOR(HGF) AND C-MET GENE IN HUMAN GASTRIC-CANCER CELL-LINES

  • Authors:
    • S FUSHIDA
    • Y YONEMURA
    • T URANO
    • A YAMAGUCHI
    • I MIYAZAKI
    • T NAKAMURA
    • H SHIKU
  • View Affiliations

  • Published online on: December 1, 1993     https://doi.org/10.3892/ijo.3.6.1067
  • Pages: 1067-1070
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Abstract

It has become evident that the hepatocyte growth factor (HGF) could be involved in the growth of various epithelial cells. In addition, it has recently been elucidated that the c-met gene, a proto-oncogene, encodes the HGF receptor. We examined the expression of HGF and c-met gene in human gastric cancer, using seven gastric cancer cell lines. The Northern blotting method revealed that, among seven cell lines used, only MKN45 expressed the c-met gene at a high efficiency, while the Southern blotting method demonstrated the amplification of the c-met gene. When the expression of HGF gene was studied by the Northern blot method, the expression of this gene was also observed only in MKN45, which was confirmed by immunostaining with polyclonal antibodies to HGF. The above results suggest the possibility that in MKN45, a gastric cancer cell line, the autocrine mechanism by HGF-met might be involved in its growth.

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December 1993
Volume 3 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
FUSHIDA S, YONEMURA Y, URANO T, YAMAGUCHI A, MIYAZAKI I, NAKAMURA T and SHIKU H: EXPRESSION OF HEPATOCYTE GROWTH FACTOR(HGF) AND C-MET GENE IN HUMAN GASTRIC-CANCER CELL-LINES. Int J Oncol 3: 1067-1070, 1993
APA
FUSHIDA, S., YONEMURA, Y., URANO, T., YAMAGUCHI, A., MIYAZAKI, I., NAKAMURA, T., & SHIKU, H. (1993). EXPRESSION OF HEPATOCYTE GROWTH FACTOR(HGF) AND C-MET GENE IN HUMAN GASTRIC-CANCER CELL-LINES. International Journal of Oncology, 3, 1067-1070. https://doi.org/10.3892/ijo.3.6.1067
MLA
FUSHIDA, S., YONEMURA, Y., URANO, T., YAMAGUCHI, A., MIYAZAKI, I., NAKAMURA, T., SHIKU, H."EXPRESSION OF HEPATOCYTE GROWTH FACTOR(HGF) AND C-MET GENE IN HUMAN GASTRIC-CANCER CELL-LINES". International Journal of Oncology 3.6 (1993): 1067-1070.
Chicago
FUSHIDA, S., YONEMURA, Y., URANO, T., YAMAGUCHI, A., MIYAZAKI, I., NAKAMURA, T., SHIKU, H."EXPRESSION OF HEPATOCYTE GROWTH FACTOR(HGF) AND C-MET GENE IN HUMAN GASTRIC-CANCER CELL-LINES". International Journal of Oncology 3, no. 6 (1993): 1067-1070. https://doi.org/10.3892/ijo.3.6.1067