Cell density-dependent regulation of tumor necrosis factor α gene expression in a human hepatoma cell line

  • Authors:
    • Shintaro Fukushima
    • Naoyuki Kaneko
    • Osamu Koiwai
    • Katsuro Koike
  • View Affiliations

  • Published online on: December 1, 2007     https://doi.org/10.3892/ijo.31.6.1485
  • Pages: 1485-1490
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Human tumor necrosis factor α (TNFα) is a pro-inflammatory cytokine expressed in many cell types. Although the TNFα gene expression in human hepatocytes has been detected previously, its regulation is not well understood yet. In this study, we demonstrated that TNFα gene expression in human hepatoma cell line, huH2-2, was activated as a function of cell density. TNFα mRNA expression was low in the low-density culture, while significantly high expression was detected in the high-density culture. Moreover, stability of TNFα mRNA was not changed by cell density, eliminating a possibility of post-transcriptional regulation. Antibody neutralization against human TNFα had no significant effect on the TNFα mRNA expression. A cellular factor for the TNFα gene expression is suggested to be accumulated in the high-density cells. Data indicate that the level of TNFα gene transcription is elevated by a cellular factor in a cell density-dependent manner without influencing the TNFα secretion under the present cell-culture conditions used.

Related Articles

Journal Cover

December 2007
Volume 31 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Fukushima S, Kaneko N, Koiwai O and Koike K: Cell density-dependent regulation of tumor necrosis factor α gene expression in a human hepatoma cell line. Int J Oncol 31: 1485-1490, 2007
APA
Fukushima, S., Kaneko, N., Koiwai, O., & Koike, K. (2007). Cell density-dependent regulation of tumor necrosis factor α gene expression in a human hepatoma cell line. International Journal of Oncology, 31, 1485-1490. https://doi.org/10.3892/ijo.31.6.1485
MLA
Fukushima, S., Kaneko, N., Koiwai, O., Koike, K."Cell density-dependent regulation of tumor necrosis factor α gene expression in a human hepatoma cell line". International Journal of Oncology 31.6 (2007): 1485-1490.
Chicago
Fukushima, S., Kaneko, N., Koiwai, O., Koike, K."Cell density-dependent regulation of tumor necrosis factor α gene expression in a human hepatoma cell line". International Journal of Oncology 31, no. 6 (2007): 1485-1490. https://doi.org/10.3892/ijo.31.6.1485