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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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February 1994 Volume 4 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 1994 Volume 4 Issue 2

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Article

CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60

  • Authors:
    • M ABOLHASSANI
    • JK TILLOTSON
    • JW CHIAO

  • View Affiliations / Copyright

    Affiliations: NEW YORK MED COLL,DEPT MED,207 VOSBURGH PAVIL,VALHALLA,NY 10595. AMER HLTH FDN,DEPT NUTR & ENDOCRINOL,VALHALLA,NY 10595.
  • Pages: 417-421
    |
    Published online on: February 1, 1994
       https://doi.org/10.3892/ijo.4.2.417
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Abstract

Release of DNA fragments into the culture supernatant of human promyelocytic cell line HL-60 was investigated. The released DNA has previously been shown to have a strong immunosuppressive effect on the proliferation of mitogen-stimulated human peripheral lymphocytes and some cancer cell lines. In this study, HL-60 cells were cultured for four days in serum-free medium and the amount of extracellular nucleic acid was monitored daily. Nucleic acids were isolated by phenol/chloroform extractions, followed by ethanol precipitations. A similar amount of DNA was identified in the HL-60 culture supernatant regardless of whether the cells were incubated for one day or up to four days. Also, the same amount of DNA was isolated from culture supernatants after washing the cells daily and reincubating into the fresh medium. During the first 8 hours of incubation no DNA material was detected in the culture supernatant, whereas at 24 h the concentration of the extracellular DNA reached a plateau. The data suggest the extracellular DNA is not accounted for totally by DNA from dying cells, and a regulatory mechanism may be involved which controls the release of DNA into the medium.

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Copy and paste a formatted citation
Spandidos Publications style
ABOLHASSANI M, TILLOTSON J and CHIAO J: CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60. Int J Oncol 4: 417-421, 1994.
APA
ABOLHASSANI, M., TILLOTSON, J., & CHIAO, J. (1994). CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60. International Journal of Oncology, 4, 417-421. https://doi.org/10.3892/ijo.4.2.417
MLA
ABOLHASSANI, M., TILLOTSON, J., CHIAO, J."CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60". International Journal of Oncology 4.2 (1994): 417-421.
Chicago
ABOLHASSANI, M., TILLOTSON, J., CHIAO, J."CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60". International Journal of Oncology 4, no. 2 (1994): 417-421. https://doi.org/10.3892/ijo.4.2.417
Copy and paste a formatted citation
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Spandidos Publications style
ABOLHASSANI M, TILLOTSON J and CHIAO J: CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60. Int J Oncol 4: 417-421, 1994.
APA
ABOLHASSANI, M., TILLOTSON, J., & CHIAO, J. (1994). CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60. International Journal of Oncology, 4, 417-421. https://doi.org/10.3892/ijo.4.2.417
MLA
ABOLHASSANI, M., TILLOTSON, J., CHIAO, J."CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60". International Journal of Oncology 4.2 (1994): 417-421.
Chicago
ABOLHASSANI, M., TILLOTSON, J., CHIAO, J."CHARACTERIZATION OF THE RELEASE OF DNA BY A HUMAN LEUKEMIA-CELL LINE HL-60". International Journal of Oncology 4, no. 2 (1994): 417-421. https://doi.org/10.3892/ijo.4.2.417
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