T-CELL RECEPTOR V-ALPHA AND V-BETA GENE USAGE IN INTERLEUKIN-2-CULTURED TUMOR-INFILTRATING LYMPHOCYTES FROM PATIENTS WITH BREAST-CANCER

  • Authors:
    • E ANDERSEN
    • J SCHOLLER
    • PT STRATEN
    • S DUUN
    • J ZEUTHEN
  • View Affiliations

  • Published online on: May 1, 1994     https://doi.org/10.3892/ijo.4.5.1035
  • Pages: 1035-1041
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Abstract

Tumor-infiltrating lymphocytes (TIL) are often found in malignant breast tumors, and have been claimed to be of prognostic value. It has been proposed that TIL may represent an enriched population of tumor-specific cytotoxic lymphocytes, reacting with antigenic determinants on the tumor cell surface through the T cell receptor (TCR) complex. We have studied the phenotype, cytotoxicity, and expression of TCR variable (V) alpha and beta chain on in vitro IL-2-cultured TIL isolated from primary malignant breast tumors from 11 patients. 10/11 cultures were dominated by CD4(+) (T-helper) cells. The different TIL cultures exhibited varying levels of cytotoxicity against the natural killer (NK)-sensitive cell line K562 and breast cancer cell line T47D. The level of clonality, as measured by PCR-based analyses of usage of the different V segments was low, as only a few tumors showed patterns of restricted V gene expression. The mean number of V alpha segments per TIL culture was higher than the number of V beta segments per culture. A significant negative correlation was observed between the number of CD4+ cells and the number of V beta segments per culture, and no other correlations between phenotypes and expression of any particular V segments were found. Neither was there any correlation between the expression of specific V alpha/V beta segments and cytotoxicity against allogeneic tumor cells.

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May 1994
Volume 4 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
ANDERSEN E, SCHOLLER J, STRATEN P, DUUN S and ZEUTHEN J: T-CELL RECEPTOR V-ALPHA AND V-BETA GENE USAGE IN INTERLEUKIN-2-CULTURED TUMOR-INFILTRATING LYMPHOCYTES FROM PATIENTS WITH BREAST-CANCER. Int J Oncol 4: 1035-1041, 1994
APA
ANDERSEN, E., SCHOLLER, J., STRATEN, P., DUUN, S., & ZEUTHEN, J. (1994). T-CELL RECEPTOR V-ALPHA AND V-BETA GENE USAGE IN INTERLEUKIN-2-CULTURED TUMOR-INFILTRATING LYMPHOCYTES FROM PATIENTS WITH BREAST-CANCER. International Journal of Oncology, 4, 1035-1041. https://doi.org/10.3892/ijo.4.5.1035
MLA
ANDERSEN, E., SCHOLLER, J., STRATEN, P., DUUN, S., ZEUTHEN, J."T-CELL RECEPTOR V-ALPHA AND V-BETA GENE USAGE IN INTERLEUKIN-2-CULTURED TUMOR-INFILTRATING LYMPHOCYTES FROM PATIENTS WITH BREAST-CANCER". International Journal of Oncology 4.5 (1994): 1035-1041.
Chicago
ANDERSEN, E., SCHOLLER, J., STRATEN, P., DUUN, S., ZEUTHEN, J."T-CELL RECEPTOR V-ALPHA AND V-BETA GENE USAGE IN INTERLEUKIN-2-CULTURED TUMOR-INFILTRATING LYMPHOCYTES FROM PATIENTS WITH BREAST-CANCER". International Journal of Oncology 4, no. 5 (1994): 1035-1041. https://doi.org/10.3892/ijo.4.5.1035