EFFECTS OF MUTANT P53 GENES ON TRANSFORMATION OF HUMAN MAMMARY EPITHELIAL-CELLS

  • Authors:
    • C BIANCO
    • G TORTORA
    • F BASOLO
    • L FIORE
    • G FONTANINI
    • G MERLO
    • DS SALOMON
    • AR BIANCO
    • F CIARDIELLO
  • View Affiliations

  • Published online on: May 1, 1994     https://doi.org/10.3892/ijo.4.5.1077
  • Pages: 1077-1082
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MCF-10A cells are a spontaneously immortalized, nontransformed human mammary epithelial cell line that contains two normal p53 alleles and produces a normal p53 protein. We have recently shown that overexpression of several genes that are important for normal mammary gland development and for neoplastic transformation, such as transforming growth factor alpha, c-Ha-rns or c-erbB-2, leads to in vitro transformation of these cells (Ciardiello et al: Mol Carcinogen 6: 43-52, 1992). To investigate the neoplastic potential of mutated forms of the p53 gene on MCF-10A cells, we have constructed two expression vector plasmids containing two p53 mutants that were isolated from human primary breast carcinomas. Overexpression of either mutant p53 gene confers on MCF-10A cells the ability to grow in serum-free medium in monolayer culture and to form colonies in semi-solid medium. Furthermore, to determine whether a mutated p53 gene may cooperate with a point mutated c-Ha-ras and/or the normal c-erbB-2 protooncogenes in the transformation of these cells, we generated clones of MCF-10A cells that overexpress a combination of these gene pruducts. Although these cells were able to grow with a higher cloning efficiency in soft agar, none of the cell lines was tumorigenic when injected subcutaneously into immunodeficient mice.

Related Articles

Journal Cover

May 1994
Volume 4 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
BIANCO C, TORTORA G, BASOLO F, FIORE L, FONTANINI G, MERLO G, SALOMON D, BIANCO A and CIARDIELLO F: EFFECTS OF MUTANT P53 GENES ON TRANSFORMATION OF HUMAN MAMMARY EPITHELIAL-CELLS. Int J Oncol 4: 1077-1082, 1994
APA
BIANCO, C., TORTORA, G., BASOLO, F., FIORE, L., FONTANINI, G., MERLO, G. ... CIARDIELLO, F. (1994). EFFECTS OF MUTANT P53 GENES ON TRANSFORMATION OF HUMAN MAMMARY EPITHELIAL-CELLS. International Journal of Oncology, 4, 1077-1082. https://doi.org/10.3892/ijo.4.5.1077
MLA
BIANCO, C., TORTORA, G., BASOLO, F., FIORE, L., FONTANINI, G., MERLO, G., SALOMON, D., BIANCO, A., CIARDIELLO, F."EFFECTS OF MUTANT P53 GENES ON TRANSFORMATION OF HUMAN MAMMARY EPITHELIAL-CELLS". International Journal of Oncology 4.5 (1994): 1077-1082.
Chicago
BIANCO, C., TORTORA, G., BASOLO, F., FIORE, L., FONTANINI, G., MERLO, G., SALOMON, D., BIANCO, A., CIARDIELLO, F."EFFECTS OF MUTANT P53 GENES ON TRANSFORMATION OF HUMAN MAMMARY EPITHELIAL-CELLS". International Journal of Oncology 4, no. 5 (1994): 1077-1082. https://doi.org/10.3892/ijo.4.5.1077