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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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June 1994 Volume 4 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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June 1994 Volume 4 Issue 6

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PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS

  • Authors:
    • Y SHIRAISHI
    • M OZAWA
    • K YAMAMOTO
    • K YAGYU
    • K NAKAYAMA
    • Y SAGARA
    • A TAKAKUSU

  • View Affiliations / Copyright

    Affiliations: KOCHI MED SCH,DEPT OBSTET & GYNECOL,MED RES LAB,NANKOKU 783,KOCHI,JAPAN. NARA MED UNIV,DEPT ANAT,KASHIHARA,NARA 634,JAPAN.
  • Pages: 1183-1189
    |
    Published online on: June 1, 1994
       https://doi.org/10.3892/ijo.4.6.1183
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Abstract

We separated characteristic mucinous ovarian cancer (OVC) antigen cells from malignantly transformed Bloom syndrome (BS) cell line (BS-SHI-4M) with the panning procedure using OVC patients sera. We undertook an immune electron-microscopic and scanning electron-microscopic study to acquire information regarding the antigenic determinant of the membrane using pre-embedding method, as well as immunofluorescence (IF) study. The distribution of Protein A colloidal gold (PAG) grains on the cell membrane of mucinous OVC antigen cells paralleled that of fluorescein-conjugated anti-human IgG observed in the IF study. The three patterns of PAG labeling of uniform labeling, uniform partial labeling, and partial labeling of one side of the cell paralleled the three patterns of IF labeling observed under IF. These findings strongly suggest the immunological reaction of BS-SHI-4M OVC-MU antigen cells with the antibody of mucinous OVC patient serum. Western blot analysis demonstrated that the antigen which characterizes mucinous OVC has a band at 84000 MW.

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Copy and paste a formatted citation
Spandidos Publications style
SHIRAISHI Y, OZAWA M, YAMAMOTO K, YAGYU K, NAKAYAMA K, SAGARA Y and TAKAKUSU A: PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. Int J Oncol 4: 1183-1189, 1994.
APA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., & TAKAKUSU, A. (1994). PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. International Journal of Oncology, 4, 1183-1189. https://doi.org/10.3892/ijo.4.6.1183
MLA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4.6 (1994): 1183-1189.
Chicago
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4, no. 6 (1994): 1183-1189. https://doi.org/10.3892/ijo.4.6.1183
Copy and paste a formatted citation
x
Spandidos Publications style
SHIRAISHI Y, OZAWA M, YAMAMOTO K, YAGYU K, NAKAYAMA K, SAGARA Y and TAKAKUSU A: PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. Int J Oncol 4: 1183-1189, 1994.
APA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., & TAKAKUSU, A. (1994). PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. International Journal of Oncology, 4, 1183-1189. https://doi.org/10.3892/ijo.4.6.1183
MLA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4.6 (1994): 1183-1189.
Chicago
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4, no. 6 (1994): 1183-1189. https://doi.org/10.3892/ijo.4.6.1183
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