PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS

  • Authors:
    • Y SHIRAISHI
    • M OZAWA
    • K YAMAMOTO
    • K YAGYU
    • K NAKAYAMA
    • Y SAGARA
    • A TAKAKUSU
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1183
  • Pages: 1183-1189
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Abstract

We separated characteristic mucinous ovarian cancer (OVC) antigen cells from malignantly transformed Bloom syndrome (BS) cell line (BS-SHI-4M) with the panning procedure using OVC patients sera. We undertook an immune electron-microscopic and scanning electron-microscopic study to acquire information regarding the antigenic determinant of the membrane using pre-embedding method, as well as immunofluorescence (IF) study. The distribution of Protein A colloidal gold (PAG) grains on the cell membrane of mucinous OVC antigen cells paralleled that of fluorescein-conjugated anti-human IgG observed in the IF study. The three patterns of PAG labeling of uniform labeling, uniform partial labeling, and partial labeling of one side of the cell paralleled the three patterns of IF labeling observed under IF. These findings strongly suggest the immunological reaction of BS-SHI-4M OVC-MU antigen cells with the antibody of mucinous OVC patient serum. Western blot analysis demonstrated that the antigen which characterizes mucinous OVC has a band at 84000 MW.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
SHIRAISHI Y, OZAWA M, YAMAMOTO K, YAGYU K, NAKAYAMA K, SAGARA Y and TAKAKUSU A: PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. Int J Oncol 4: 1183-1189, 1994.
APA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., & TAKAKUSU, A. (1994). PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS. International Journal of Oncology, 4, 1183-1189. https://doi.org/10.3892/ijo.4.6.1183
MLA
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4.6 (1994): 1183-1189.
Chicago
SHIRAISHI, Y., OZAWA, M., YAMAMOTO, K., YAGYU, K., NAKAYAMA, K., SAGARA, Y., TAKAKUSU, A."PANNING AND IMMUNE ELECTRON-MICROSCOPIC STUDY OF MUCINOUS OVARIAN-CANCER ANTIGEN-SPECIFIC CELLS DERIVED FROM MALIGNANTLY TRANSFORMED BLOOM-SYNDROME B-LYMPHOBLASTOID CELLS". International Journal of Oncology 4, no. 6 (1994): 1183-1189. https://doi.org/10.3892/ijo.4.6.1183