EFFECT OF ESCHERICHIA-COLI THIOREDOXIN, A HOMOLOG TO THE ADULT T-CELL LEUKEMIA-DERIVED FACTOR, ON EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES

  • Authors:
    • A BAZZICHI
    • M INCAPRERA
    • C GARZELLI
  • View Affiliations

  • Published online on: July 1, 1994     https://doi.org/10.3892/ijo.5.1.41
  • Pages: 41-46
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In order to investigate the role of autocrine growth factors on cell growth of Epstein-Barr virus (EBV)-transformed human B lymphocytes, we have studied the effect of E. coli recombinant thioredoxin (r-thioredoxin), a homologue of adult-T-cell leukemia-derived factor (ADF), on the cell growth of various human lymphoid cell lines, either EBV-positive or -negative. It was found that, in serum-free culture conditions, exogenous r-thioredoxin increases DNA synthesis in lymphoblastoid and Burkitt's cell lines, independently of EBV genome expression; moreover, the combined effect of r-thioredoxin and interleukin-I (IL-1) or IL-6 resulted in a marked increase in DNA synthesis in lymphoblastoid cells, but not in Burkitt's cell lines. Anti r-thioredoxin monoclonal antibodies were developed and used to test the possibility of interfering with r-thioredoxin-induced cell proliferation. It was found that the growth-promoting activity of r-thioredoxin was inhibited by an anti-r-thioredoxin monoclonal antibody in a dose-dependent manner in P3HR-1 cells, a Burkitt's lymphoma cell line harboring an EBNA-2-defective virus, but not in other lymphoid cell lines, thus indicating that target cells may play an active role in antibody-mediated thioredoxin neutralization.

Related Articles

Journal Cover

July 1994
Volume 5 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
BAZZICHI A, INCAPRERA M and GARZELLI C: EFFECT OF ESCHERICHIA-COLI THIOREDOXIN, A HOMOLOG TO THE ADULT T-CELL LEUKEMIA-DERIVED FACTOR, ON EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES. Int J Oncol 5: 41-46, 1994
APA
BAZZICHI, A., INCAPRERA, M., & GARZELLI, C. (1994). EFFECT OF ESCHERICHIA-COLI THIOREDOXIN, A HOMOLOG TO THE ADULT T-CELL LEUKEMIA-DERIVED FACTOR, ON EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES. International Journal of Oncology, 5, 41-46. https://doi.org/10.3892/ijo.5.1.41
MLA
BAZZICHI, A., INCAPRERA, M., GARZELLI, C."EFFECT OF ESCHERICHIA-COLI THIOREDOXIN, A HOMOLOG TO THE ADULT T-CELL LEUKEMIA-DERIVED FACTOR, ON EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES". International Journal of Oncology 5.1 (1994): 41-46.
Chicago
BAZZICHI, A., INCAPRERA, M., GARZELLI, C."EFFECT OF ESCHERICHIA-COLI THIOREDOXIN, A HOMOLOG TO THE ADULT T-CELL LEUKEMIA-DERIVED FACTOR, ON EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES". International Journal of Oncology 5, no. 1 (1994): 41-46. https://doi.org/10.3892/ijo.5.1.41