FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS

KAMIHIRA,S; MATUTES,E; MARCO,JG; HODA,S; MORILLA,R; OWUSU-ANKOMAH,K; CROWFORD,A; ELLIS,J; CATOVSKY,D

doi:10.3892/ijo.5.2.211

August 1994 Volume 5 Issue 2

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August 1994 Volume 5 Issue 2

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Article

FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS

Authors:
- S KAMIHIRA
- E MATUTES
- JG MARCO
- S HODA
- R MORILLA
- K OWUSU-ANKOMAH
- A CROWFORD
- J ELLIS
- D CATOVSKY
View Affiliations / Copyright

Affiliations: INST CANC RES,LONDON SW3 6JJ,ENGLAND. ROYAL MARSDEN HOSP,ACAD DEPT HAEMATOL & CYTOGENET,LONDON SW3 6JJ,ENGLAND.
Pages: 211-214
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Published online on: August 1, 1994

https://doi.org/10.3892/ijo.5.2.211
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Abstract

The relative DNA content of peripheral blood cells from 79 cases with lymphoid leukemias was analyzed by a dual-parameter flow cytometric analysis. The leukemia samples corresponded to: chronic lymphocytic leukemia (CLL) 40, CLL with more than 10% prolymphocytes (CLL/PL) 12, CLL mixed 9, prolymphocytic leukemia (PLL) 5, and B-cell lymphoma in leukemic phase 13. DNA aneuploidy was found overall in 26 (32.9%) of the cases and these corresponded to: 7 (17.5%) with CLL, 7 (58.3%) with CLL/PL, 4 (44.4%) with CLL mixed, 2 (40%) with PLL and 6 (46.2%) with B-cell lymphoma. There was a good correlation between DNA content and cytogenetics/fluorescent in situ hybridization in all but 2 cases as follows: 6 of 7 cases with diploid DNA had normal karyotype and only one had trisomy 12: 4 of 6 cases with hyperdiploid DNA had trisomy 12, one had tetraploidy and only one had a normal karyotype. Two cases were hypodiploid both by DNA and cytogenetic analysis. Our findings demonstrate a higher incidence of DNA aneuploidy in B-cell lymphoma in leukemic phase, PLL, and atypical CLL in comparison with typical CLL and a good correlation with cytogenetics. We conclude that flow cytometric DNA analysis represents a useful, sensitive, and rapid method to detect and monitor minimal changes of DNA content in leukemic lymphocytes without the need of short-term cultures.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS

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