FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS

  • Authors:
    • S KAMIHIRA
    • E MATUTES
    • JG MARCO
    • S HODA
    • R MORILLA
    • K OWUSU-ANKOMAH
    • A CROWFORD
    • J ELLIS
    • D CATOVSKY
  • View Affiliations

  • Published online on: August 1, 1994     https://doi.org/10.3892/ijo.5.2.211
  • Pages: 211-214
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The relative DNA content of peripheral blood cells from 79 cases with lymphoid leukemias was analyzed by a dual-parameter flow cytometric analysis. The leukemia samples corresponded to: chronic lymphocytic leukemia (CLL) 40, CLL with more than 10% prolymphocytes (CLL/PL) 12, CLL mixed 9, prolymphocytic leukemia (PLL) 5, and B-cell lymphoma in leukemic phase 13. DNA aneuploidy was found overall in 26 (32.9%) of the cases and these corresponded to: 7 (17.5%) with CLL, 7 (58.3%) with CLL/PL, 4 (44.4%) with CLL mixed, 2 (40%) with PLL and 6 (46.2%) with B-cell lymphoma. There was a good correlation between DNA content and cytogenetics/fluorescent in situ hybridization in all but 2 cases as follows: 6 of 7 cases with diploid DNA had normal karyotype and only one had trisomy 12: 4 of 6 cases with hyperdiploid DNA had trisomy 12, one had tetraploidy and only one had a normal karyotype. Two cases were hypodiploid both by DNA and cytogenetic analysis. Our findings demonstrate a higher incidence of DNA aneuploidy in B-cell lymphoma in leukemic phase, PLL, and atypical CLL in comparison with typical CLL and a good correlation with cytogenetics. We conclude that flow cytometric DNA analysis represents a useful, sensitive, and rapid method to detect and monitor minimal changes of DNA content in leukemic lymphocytes without the need of short-term cultures.

Related Articles

Journal Cover

August 1994
Volume 5 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
KAMIHIRA S, MATUTES E, MARCO J, HODA S, MORILLA R, OWUSU-ANKOMAH K, CROWFORD A, ELLIS J and CATOVSKY D: FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS. Int J Oncol 5: 211-214, 1994
APA
KAMIHIRA, S., MATUTES, E., MARCO, J., HODA, S., MORILLA, R., OWUSU-ANKOMAH, K. ... CATOVSKY, D. (1994). FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS. International Journal of Oncology, 5, 211-214. https://doi.org/10.3892/ijo.5.2.211
MLA
KAMIHIRA, S., MATUTES, E., MARCO, J., HODA, S., MORILLA, R., OWUSU-ANKOMAH, K., CROWFORD, A., ELLIS, J., CATOVSKY, D."FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS". International Journal of Oncology 5.2 (1994): 211-214.
Chicago
KAMIHIRA, S., MATUTES, E., MARCO, J., HODA, S., MORILLA, R., OWUSU-ANKOMAH, K., CROWFORD, A., ELLIS, J., CATOVSKY, D."FLOW-CYTOMETRY DETECTION OF MINIMAL DNA ANEUPLOIDY IN MATURE LYMPHOID LEUKEMIAS - COMPARISON WITH METAPHASE AND INTERPHASE CYTOGENETICS". International Journal of Oncology 5, no. 2 (1994): 211-214. https://doi.org/10.3892/ijo.5.2.211