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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 1994 Volume 5 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 1994 Volume 5 Issue 4

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Article

INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS

  • Authors:
    • W UCKERT
    • O ASCHE
    • B MAHANTY
    • I FICHTNER

  • View Affiliations / Copyright

    Affiliations: Affliations not specified
  • Pages: 865-871
    |
    Published online on: October 1, 1994
       https://doi.org/10.3892/ijo.5.4.865
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Abstract

The human cytomegalovirus immediate early promoter (CMV), the herpes simplex virus thymidine kinase promoter (TK), and the viral LTR promoter were tested as transcriptional control elements for the expression of the human tumor necrosis factor alpha (TNF) gene in a Moloney murine leukemia virus-based vector. Virus vector particles generated in the amphotropic packaging cell line PA317 were used to infect human colon adenocarcinoma cells LS174T. The strength of the different promoters was compared based on the amount of biologically active TNF produced by LS174T cells. Furthermore, the influence of the amount of secreted cytokine on growth characteristics of LS174T cells in vitro and on tumor growth in nude mice was analysed. Among different cell clones tested, the CMV-promoter was considerably stronger than the TK- and the viral LTR-promoter. However, despite of the amount of TNF produced by the tumor cells, individual LS174T cell clones were characterized by a similar growth inhibition of 65 to 80% in comparison to parental tumor cells or cells infected with the non-recombinat viral vector. When TNF-producing tumor cells were injected into nude mice, tumors were completely rejected only in case of CMV-promoted TNF-expression while only partial growth inhibition was obtained in case of TK- and LTR-promoted TNF-expression.

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Copy and paste a formatted citation
Spandidos Publications style
UCKERT W, ASCHE O, MAHANTY B and FICHTNER I: INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS. Int J Oncol 5: 865-871, 1994.
APA
UCKERT, W., ASCHE, O., MAHANTY, B., & FICHTNER, I. (1994). INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS. International Journal of Oncology, 5, 865-871. https://doi.org/10.3892/ijo.5.4.865
MLA
UCKERT, W., ASCHE, O., MAHANTY, B., FICHTNER, I."INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS". International Journal of Oncology 5.4 (1994): 865-871.
Chicago
UCKERT, W., ASCHE, O., MAHANTY, B., FICHTNER, I."INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS". International Journal of Oncology 5, no. 4 (1994): 865-871. https://doi.org/10.3892/ijo.5.4.865
Copy and paste a formatted citation
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Spandidos Publications style
UCKERT W, ASCHE O, MAHANTY B and FICHTNER I: INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS. Int J Oncol 5: 865-871, 1994.
APA
UCKERT, W., ASCHE, O., MAHANTY, B., & FICHTNER, I. (1994). INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS. International Journal of Oncology, 5, 865-871. https://doi.org/10.3892/ijo.5.4.865
MLA
UCKERT, W., ASCHE, O., MAHANTY, B., FICHTNER, I."INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS". International Journal of Oncology 5.4 (1994): 865-871.
Chicago
UCKERT, W., ASCHE, O., MAHANTY, B., FICHTNER, I."INFLUENCE OF DIFFERENT PROMOTERS ON THE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA IN LS174T HUMAN COLON-CARCINOMA CELLS". International Journal of Oncology 5, no. 4 (1994): 865-871. https://doi.org/10.3892/ijo.5.4.865
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