AUTONOMOUS REPLICATION ACTIVITY OF A HUMAN MITOCHONDRIAL-DNA SEQUENCE INSERTED INTO GENOMIC DNA
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- Published online on: November 1, 1994 https://doi.org/10.3892/ijo.5.5.1003
- Pages: 1003-1008
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Abstract
Mitochondrial DNA (mtDNA) fragments can be found inserted into nuclear DNA and may contribute to cancer and aging. A HeLaTG-cell nuclear transcript was shown to include human cytochrome oxidase subunit 3 (coxIII) mtDNA fused to c-myc sequences. Independently, a coxIII-containing cDNA was discovered to replicate autonomously in HeLa cells. We show that the HeLaTG mtDNA insertion has autonomous replication activity when transfected into HeLa cells and in a mammalian in vitro replication system. Replication in vitro starts within the fusion cDNA sequence. Insertion of an element which could function as a replication origin may affect cellular DNA replication and proliferation.
