INDUCTION OF RESISTANCE TO DAUNORUBICIN IN DRUG-SENSITIVE LEUKEMIA P388 CELLS - A ROLE OF PKC-BETA-I ISOZYME

  • Authors:
    • S GOLLAPUDI
    • J DENIEGA
    • V SONI
    • S GUPTA
  • View Affiliations

  • Published online on: December 1, 1994     https://doi.org/10.3892/ijo.5.6.1227
  • Pages: 1227-1231
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A role of protein kinase C (PKC) has been suggested in multidrug resistance (MDR). Because of the molecular and biochemical heterogeneity of PKC, we examined a role of PKC beta isozyme in drug sensitive murine leukemia P388 cell line. Drug sensitive P388 and MDR P388/ADR cells were treated with various concentrations of 12-deoxyphorbol-13-O-phenylacetate 20 acetate (DPPA, an agonist of PKC beta I isozyme) and examined for its effect on daunorubicin (DNR) accumulation and sensitivity to DNR. dPPA increased DNR resistance and decreased DNR accumulation in P388 cells but had no effect in P388/ADR cells. The reduced dPPA-induced DNR accumulation was due to decreased uptake without any effect on DNR efflux. Furthermore, treatment of P388 cells with dPPA was associated with translocation of PKC beta isozyme from cytosol to plasma membrane. These data suggest that PKC beta I isozyme plays a role in acquired drug resistance.

Related Articles

Journal Cover

December 1994
Volume 5 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
GOLLAPUDI S, DENIEGA J, SONI V and GUPTA S: INDUCTION OF RESISTANCE TO DAUNORUBICIN IN DRUG-SENSITIVE LEUKEMIA P388 CELLS - A ROLE OF PKC-BETA-I ISOZYME. Int J Oncol 5: 1227-1231, 1994.
APA
GOLLAPUDI, S., DENIEGA, J., SONI, V., & GUPTA, S. (1994). INDUCTION OF RESISTANCE TO DAUNORUBICIN IN DRUG-SENSITIVE LEUKEMIA P388 CELLS - A ROLE OF PKC-BETA-I ISOZYME. International Journal of Oncology, 5, 1227-1231. https://doi.org/10.3892/ijo.5.6.1227
MLA
GOLLAPUDI, S., DENIEGA, J., SONI, V., GUPTA, S."INDUCTION OF RESISTANCE TO DAUNORUBICIN IN DRUG-SENSITIVE LEUKEMIA P388 CELLS - A ROLE OF PKC-BETA-I ISOZYME". International Journal of Oncology 5.6 (1994): 1227-1231.
Chicago
GOLLAPUDI, S., DENIEGA, J., SONI, V., GUPTA, S."INDUCTION OF RESISTANCE TO DAUNORUBICIN IN DRUG-SENSITIVE LEUKEMIA P388 CELLS - A ROLE OF PKC-BETA-I ISOZYME". International Journal of Oncology 5, no. 6 (1994): 1227-1231. https://doi.org/10.3892/ijo.5.6.1227