CARCINOMA-CELLS EXPRESS IL-8 AND THE IL-8 RECEPTOR - THEIR INHIBITION ATTENUATES THE GROWTH OF CARCINOMA-CELLS

  • Authors:
    • T ISHIKO
    • K SAKAMOTO
    • SI YAMASHITA
    • Y MASUDA
    • H KAMOHARA
    • S MITA
    • M HIRASHIMA
    • M OGAWA
  • View Affiliations

  • Published online on: January 1, 1995     https://doi.org/10.3892/ijo.6.1.119
  • Pages: 119-122
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We investigated the expression of IL-8 and the IL-8 receptor (IL-8R) in human carcinoma cells and the role of IL-8 and IL-8R in the growth of carcinoma cells. IL-8 mRNA was detected in 16 of 20 (80%) carcinoma cell lines and 20 of 24 (83.3%) cancer tissues by Northern blot analysis. IL-8R mRNA was expressed in 7 of 11 (63.6%) carcinoma cell lines by reverse transcriptase polymerase chain reaction (RT-PCR). Neutrophil chemotactic activity in the culture supernatant of carcinoma cell lines correlated with immunoreactive IL-X concentration. Growth of carcinoma cells was significantly inhibited in the presence of anti-IL-8 antibody or IL-8R antisense oligonucleotide. These results revealed that IL-8 and IL-8R are expressed in the majority of carcinoma cells and suggest that they might play a role in the growth of carcinoma cells.

Related Articles

Journal Cover

January 1995
Volume 6 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
ISHIKO T, SAKAMOTO K, YAMASHITA S, MASUDA Y, KAMOHARA H, MITA S, HIRASHIMA M and OGAWA M: CARCINOMA-CELLS EXPRESS IL-8 AND THE IL-8 RECEPTOR - THEIR INHIBITION ATTENUATES THE GROWTH OF CARCINOMA-CELLS. Int J Oncol 6: 119-122, 1995
APA
ISHIKO, T., SAKAMOTO, K., YAMASHITA, S., MASUDA, Y., KAMOHARA, H., MITA, S. ... OGAWA, M. (1995). CARCINOMA-CELLS EXPRESS IL-8 AND THE IL-8 RECEPTOR - THEIR INHIBITION ATTENUATES THE GROWTH OF CARCINOMA-CELLS. International Journal of Oncology, 6, 119-122. https://doi.org/10.3892/ijo.6.1.119
MLA
ISHIKO, T., SAKAMOTO, K., YAMASHITA, S., MASUDA, Y., KAMOHARA, H., MITA, S., HIRASHIMA, M., OGAWA, M."CARCINOMA-CELLS EXPRESS IL-8 AND THE IL-8 RECEPTOR - THEIR INHIBITION ATTENUATES THE GROWTH OF CARCINOMA-CELLS". International Journal of Oncology 6.1 (1995): 119-122.
Chicago
ISHIKO, T., SAKAMOTO, K., YAMASHITA, S., MASUDA, Y., KAMOHARA, H., MITA, S., HIRASHIMA, M., OGAWA, M."CARCINOMA-CELLS EXPRESS IL-8 AND THE IL-8 RECEPTOR - THEIR INHIBITION ATTENUATES THE GROWTH OF CARCINOMA-CELLS". International Journal of Oncology 6, no. 1 (1995): 119-122. https://doi.org/10.3892/ijo.6.1.119