ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS

  • Authors:
    • R BERNARDI
    • C NEGRI
    • M DONZELLI
    • F GUANO
    • AI SCOVASSI
  • View Affiliations

  • Published online on: January 1, 1995     https://doi.org/10.3892/ijo.6.1.203
  • Pages: 203-208
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Abstract

We have studied the phosphorylation of DNA topoisomerases II in HeLa cells focusing on the beta isoform of the enzyme which is very difficult to analyze because of its instability. In proliferating cells, we observed that both the a and beta isozymes are labeled after cell incubation with (32)p. The phosphorylation of beta enzyme occurs to a low extent, thus reflecting the expression of topoisomerases II during cell cycle. In cells treated with etoposide, the activity of topoisomerase II is inhibited and the level of phosphorylation decreases, suggesting a possible cooperation between this modification and drug response.

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January 1995
Volume 6 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
BERNARDI R, NEGRI C, DONZELLI M, GUANO F and SCOVASSI A: ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS. Int J Oncol 6: 203-208, 1995.
APA
BERNARDI, R., NEGRI, C., DONZELLI, M., GUANO, F., & SCOVASSI, A. (1995). ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS. International Journal of Oncology, 6, 203-208. https://doi.org/10.3892/ijo.6.1.203
MLA
BERNARDI, R., NEGRI, C., DONZELLI, M., GUANO, F., SCOVASSI, A."ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS". International Journal of Oncology 6.1 (1995): 203-208.
Chicago
BERNARDI, R., NEGRI, C., DONZELLI, M., GUANO, F., SCOVASSI, A."ANALYSIS OF PHOSPHORYLATION LEVEL OF DNA TOPOISOMERASE-II-ALPHA AND TOPOISOMERASE-II-BETA IN HUMAN HELA-CELLS". International Journal of Oncology 6, no. 1 (1995): 203-208. https://doi.org/10.3892/ijo.6.1.203