MECHANISMS OF KAPOSIS-SARCOMA CELL SUPERNATANT-INDUCED VASCULAR CELL INVASION

  • Authors:
    • D GIUNCIUGLIO
    • R BENELLI
    • L MASIELLO
    • I PAGLIERI
    • A PESARINI
    • M PRESTA
    • DM NOONAN
    • A ALBINI
  • View Affiliations

  • Published online on: March 1, 1995     https://doi.org/10.3892/ijo.6.3.539
  • Pages: 539-546
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Abstract

Kaposi's sarcoma (KS) is a highly angiogenic lesion frequently associated with acquired immune deficiency syndrome. Histologically the lesions appear to contain proliferative 'spindle shaped' cells with a mixed smooth muscle-endothelial-fibroblastic histotype and a conspicuous neovascularization, derived from host cell recruitment. Media conditioned by cultured KS cells (KS-CM) have angiogenic properties. KS-CM is able to promote endothelial and smooth muscle cell migration and invasion. The mechanisms of this KS-CM activity are still unknown. We hypothesize that KS-CM contains numerous factors with different roles in inducing the neo angiogenic process. We show that AIDS-IST-KS cell supernatants induce gelatinase A production and plasminogen activator (PA) up-regulation in vascular cells. KS-CM activity in vivo is heparin dependent. Also bFGF alone, a heparin dependent factor, alone can induce endothelial and smooth muscle cell invasion, MMP-2 production and PA activity. However, antibodies to bFGF do not block KS-CM activity and do not reduce the effect on PA up-regulation. This evidence suggests that heparin-binding factors other than bFGF may be present. Chromatography of KS-CM on heparin-sepharose demonstrates the presence of two heparin-binding fractions with chemotactic and gelatinase A inducing activity. The flow through was also active. KS-CM absorption on heparin-sepharose beads did not modify its induction of PA activity, further evidence for the presence of non heparin-binding factors as well.

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March 1995
Volume 6 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
GIUNCIUGLIO D, BENELLI R, MASIELLO L, PAGLIERI I, PESARINI A, PRESTA M, NOONAN D and ALBINI A: MECHANISMS OF KAPOSIS-SARCOMA CELL SUPERNATANT-INDUCED VASCULAR CELL INVASION. Int J Oncol 6: 539-546, 1995
APA
GIUNCIUGLIO, D., BENELLI, R., MASIELLO, L., PAGLIERI, I., PESARINI, A., PRESTA, M. ... ALBINI, A. (1995). MECHANISMS OF KAPOSIS-SARCOMA CELL SUPERNATANT-INDUCED VASCULAR CELL INVASION. International Journal of Oncology, 6, 539-546. https://doi.org/10.3892/ijo.6.3.539
MLA
GIUNCIUGLIO, D., BENELLI, R., MASIELLO, L., PAGLIERI, I., PESARINI, A., PRESTA, M., NOONAN, D., ALBINI, A."MECHANISMS OF KAPOSIS-SARCOMA CELL SUPERNATANT-INDUCED VASCULAR CELL INVASION". International Journal of Oncology 6.3 (1995): 539-546.
Chicago
GIUNCIUGLIO, D., BENELLI, R., MASIELLO, L., PAGLIERI, I., PESARINI, A., PRESTA, M., NOONAN, D., ALBINI, A."MECHANISMS OF KAPOSIS-SARCOMA CELL SUPERNATANT-INDUCED VASCULAR CELL INVASION". International Journal of Oncology 6, no. 3 (1995): 539-546. https://doi.org/10.3892/ijo.6.3.539